【Objective】 To prepare chitosan-polyethylene glycol nanoparticles(CS-PEG) carrying gene and study its characteristics in vitro.【Methods】 CS-PEG was synthesized through stem grafting and copolymerizing.The nanoparticles carrying gene were prepared by complex coacervation.The shape,size,zeta electric potential,envelopment rate and carry gene rate were inspected to evaluate characteristics of the vector and effect of gene transfection was detected.The expression of [...]
18
May
18
May
Diagnosis and treatment for gastrointestinal tract stromal tumors of small intestine
Objective To review the diagnosis,treatment and prognosis of gastrointestinal stromal tumors(GIST) of the small intestine.Methods The clinicopathological and follow-up data of 25 patients with GIST of the small intestine admitted to Cancer Hospital during March 1999 to May 2006 were analyzed retrospectively.Results The pathological examination showed malignant lesions in 23 cases(92.0%),junctional lesions in 2 cases(8.0%).The [...]
18
May
Effect of bone sialoprotien on PI3K-AKT signaling pathway of breast cancer MDA-MB-231 cells
Objective: To investigate the effect of bone sialoprotein(BSP) on the PI3K-AKT signaling pathway in breast cancer MDA-MB-231 cells.Methods: BSP-silenced MDA-MB-231 cells were treated with recombinant human BSP(rhBSP) and the PI3K-AKT specific inhibitor LY294002.Western blotting analysis was used to detect the phosphorylation of AKT,qPCR was conducted to evaluate caspase-3,cyclin D1 mRNA expressions,and the proliferation of cells [...]
18
May
Levels of CD4+CD25+Regulatory T Cells & Expression of FOXP3 mRNA after Operation in Peripheral Blood from Patients with Prostate Cancer and its Clinical Signifi
Objective To explore the rate change of CD4+ CD25+ regulatory T cells and expression of FOXP3 mRNA after operation in peripheral blood from patients with prostate cancer and its clinical significance.Methods Flow cytometry was used to analyze the proportion of CD4+CD25+ regulatory T cells/CD4+T cells in peripheral blood mononuclear cells(PBMC) among 50 patients with prostate [...]
18
May
Expressions of apoptosis-associated genes PDCD5 in Uygur and Han in Xinjiang in Cervix cancer and its clinical significance
Objective:To evaluate the expression and clinical signifcance of PDCD5 in Uygur and Han in Xinjiang in Cervix cancer. Methods:Using immunohistochemistry used to detect the expression of PDCD5 in 42 patients with Cervix cancer(Uygur 18 cases,24 cases of the Han). Results:There were the expressions of PDCD5 in Uygur and Han no significant difference(P>0.05). Conclusion:⑴the expressions of [...]
18
May
The expression and significance of thyroid hormone in nonmaglinant-maglinant breast diseases
Objective:To observe the expression and significance of thyroid hormone in nonmaglinant-maglinant breast disease.Methods:The expression of thyroid hormone(T3,FT3,T4,FT4) was assayed by electrochemiluminescence in breast cancer,breast fibroadenoma,breast hyperplasia and normal subjects.Results:The expression of T3,T4 and FT4 is nonsense between breast cancer,breast fibroadenoma,breast hyperplasia and normal subject(P>0.05).The expression of FT3 is significantly different(P<0.001),expression of FT3 is significantly higher [...]
17
May
The effect of sorafenib on growth,apoptosis and P-ERK expression in human gastric cancer SGC-7901 cells
Objective To investigate the multiple molecular targeted agent,sorafenib,in human gastric cancer SGC-7901 cell proliferation,apoptosis and P-ERK expression,and explore its possible mechanism.Methods MTT method was used to detect antiproliferative ratio of sorafenib on human gastric cancer SGC-7901 cell;immunocytochemical method for detection of gastric cancer cells P-ERK protein expression;and flow cytometry to analyze gastric cancer cell apoptosis.Results [...]
17
May
Application of fast-track surgery in high-risk colorectal cancer patients
Objective: To discuss the safety of high-risk colorectal cancer with fast-track surgery.Methods: From November 2007 to September 2008,264 high-risk colorectal cancer patients were analyzed retrospectively.Postoperative early rehabilitations and implementation of fast-track rectal surgery were studied and compared.Results: In early rehabilitation,first time of ambulation,oral intake,urinary catheter and postoperative hospital stay of FT group were significantly earlier [...]
17
May
THE SIGNIFICANCE OF ABCG2 EXPRESSION ON ADRENOCORTICAL CARCINOMA IN VARIOUS MICROENVIRONMENT
Objective:To investigate the ABCG2 expression on adrenocortical carcinoma in various microenvironment.Methods:Adrenocortical carcinoma cell line(SW-13) cells were used to established different microenvironment in vitro and in vivo,the tissue and cells of above mention were detected with flow cytometry.Results:The expression of ABCG2 on SW-13 cells in metastasis lymph [(5.41±2.57)%] was higher than that in standard culture medium [...]
17
May