OBJECTIVE:To investigate the different expressions of Survivin,VEGF,Smac/DIABLO and their correlation in metastatic ovarian cancer originated from gastric carcinoma and primary ovarian cancer.METHODS:Immunochemistry and flow cytometry were used to investigate the expressions of Survivin,VEGF and Smac/DIABLO proteins in normal ovarian epithelium,benign and epithelial ovarian tumor,metastatic ovarian cancer originated from gastric carcinoma,primary epithelial ovarian cancer,and their relationship [...]
18
May
18
May
Effects of interference of XIAP by siRNA on the biological behaviors in ovarian carcinoma SKOV-3 cells
OBJECTIVE:To silence the expression of XIAP in ovarian cancer cell line SKOV-3 by using small interfering RNA(siRNA),and observe its effects on the proliferation and apoptosis.METHODS:The XIAP siRNA was transfected into SKOV-3 cells and the cell proliferation was evaluated using MTT assay.The apoptosis rate and cell cycle distribution were analyzed using Annexin-V and flow cytometry methods.RESULTS:XIAP [...]
18
May
Association study between single nucleotide polymorphism of BRCA2 and susceptibility to sporadic breast cancer in Han Chinese women from Jiangxi Province
OBJECTIVE:To evaluate the relationship between single nucleotide polymorphisms(SNPs) of BRCA2 and susceptibility to sporadic breast cancer in Han Chinese women from Jiangxi Province.METHODS:Three single nucleotide polymorphisms(rs766173,rs144848,rs1801426) from the coding regions of BRCA2 were detected by Sequenom MassArray○R iPLEX GOLD System in 152 sporadic breast cancer patients and 165 healthy controls collected from the First Affiliated [...]
18
May
18
May
Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Detected the Single Nucleotide Polymorphisms of MiR146a
Objective:Using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS) analysis to detect the single nucleotide polymorphisms of miR146a,and explorer the relationship between Hazakh’s esophageal carcinoma and the single nucleotide polymorphisms of miR146a gene. Methods:Using MALDI-TOF MS to detect the SNP of 261 cases of Hazakh’s esophageal squamous cell carcinomas(ESCC),190 cases of cancer-free controls in [...]
18
May
Study of recombinant adenovirus mediated hyrdC gene effect on gastric cancer SGC-7901 cell line
Objective To observe the effect of recombinant adenovirus mediated hyrdC gene effect on gastric cancer cell line.Methods Transfected the constructed Ad-hyrdC recombinant adenovirus to gastric cancer SGC-7901 cell line,determined the expression of hyrdC protein in the cell line by immunohistochemistry,investigated and constrasted the differences of cell growth between each groups by MTT and cell growth [...]
18
May
Effects of Pak1 siRNA to the growth of gastric cancer cell BGC823
Objective To observe the effects of Pak1 siRNA to cell growth and cell cycle of gastric cancer cell BGC823.Methods The expression of Pak1 in gastric cancer cell was detected by Western blot.Pak1 siRNA was transfected into BGC823 cell.The growth ability of BGC823 cell was observed by MTT assay and counting cell number,and the cell cycle [...]
17
May
Study on expressions of survivin and p53 in epithelial ovarian cancer
Objective To explore the expression of survivin and p53 in epithelial ovarian cancer and their clinical significances.Methods Expression intensity of survivin and p53 were detected by immunohistochemistry in the tissues of 46 cases with epithelial ovarian cancer,the relationship between clinical and pathological characteristics with survivin and p53 expressions were analysed.The 44 cases with benign epithelial [...]
17
May
Impact of comprehensive nursing intervention on the quality of life of patients with laryngeal cancer
Objective:To evaluate the impact of comprehensive nursing intervention on the quality of life of patients with laryngeal cancer.Methods:87 patients with laryngeal cancer were randomly divided into experimental group(n=45) and control group(n=42).The routine nursing care was given to the patients in the control group and the comprehensive nursing intervention was applied to the patients in the [...]
17
May
Clinical effect of thin-prep cell test and high-risk HPV in diagnosis of intraepithelial neoplasia and cervical cancer
Objective To study the clinical application value of screening cervical precancerous lesions of high risk human papillomavirus(HR-HPV) testing combined with thin-prep cell test,(TCT) in prevention of cervical lesions and cervical cancer.Methods Totally 763 women in our hospital from Jan 2006 to Jan 2010,were screened by HR-HPV test or thin-prep cell test.Directed biopsies under colposcopy were [...]