Objective:To study the relationship of CHFR CpG island methylation status on proliferation and apoptosis of esophageal cancer Eca109 cells.Methods: Eca109 cells were treated with different concentrations(2,5,and 10 μmol/L) of 5-Aza-2′-deoxycytidine(5-Aza-CdR),and untreated Eca109 cells were used as control.Methylation-specific PCR(MSP) analysis was used to evaluate the CpG island methylation status of CHFR gene,and RT-PCR was used to [...]
18
May
18
May
Effect of JNK signaling pathway inhibition on TRAIL-induced apoptosis in human gastric cancer cells
OBJECTIVE:To investigate the effect of JNK signaling pathway on TRAIL-induced apoptosis in human gastric cancer cells.METHODS:Methyl thiazolyl tetrazolium(MTT) assay was used to determine the cell viability;Western blotting was used to detect the levels of phosphor-JNK and total JNK;Flow cytometry following Propidium Iodide(PI) staining was used to determine the apoptosis as well as cell cycle distribution.The [...]
18
May
Effects of interference of XIAP by siRNA on the biological behaviors in ovarian carcinoma SKOV-3 cells
OBJECTIVE:To silence the expression of XIAP in ovarian cancer cell line SKOV-3 by using small interfering RNA(siRNA),and observe its effects on the proliferation and apoptosis.METHODS:The XIAP siRNA was transfected into SKOV-3 cells and the cell proliferation was evaluated using MTT assay.The apoptosis rate and cell cycle distribution were analyzed using Annexin-V and flow cytometry methods.RESULTS:XIAP [...]
17
May
The effect of sorafenib on growth,apoptosis and P-ERK expression in human gastric cancer SGC-7901 cells
Objective To investigate the multiple molecular targeted agent,sorafenib,in human gastric cancer SGC-7901 cell proliferation,apoptosis and P-ERK expression,and explore its possible mechanism.Methods MTT method was used to detect antiproliferative ratio of sorafenib on human gastric cancer SGC-7901 cell;immunocytochemical method for detection of gastric cancer cells P-ERK protein expression;and flow cytometry to analyze gastric cancer cell apoptosis.Results [...]
17
May
Tubeimoside 1 enhances chemosensitivity of drug-resistant ovarian cancer A2780/DDP cells to cisplatin
Objective To determine the effect of tubeimoside 1(TBMS1) in enhancing chemosensitivity of drug-resistant ovarian cancer cell line(A2780/DDP) to cisplatin.MethodsThe A2780/DDP cells were treated with different concentrations of cisplatin and TBMS1 to analyze 50% inhibiting concentration(IC50) by methyl thiazolyl tetrazolium(MTT) assay.Experiment was divided into 4 groups.Control group:treatment with physiological saline;cisplatin group:treatment with 6 μmol/L cisplatin;Combination of [...]
17
May
Effect and mechanism of proliferation and apoptosis induced by arctigenin in human hepatocellular carcinoma SMMC-7721 cell
Objective To evaluate the effect of proliferation and apoptosis induced by arctigenin(ARG) in human hepatocellular carcinoma SMMC-7721 cell,and explore its mechanism.Methods SMMC-7721 cell were treated with different concentration ARG.Proliferation and apoptosis of SMMC-7721 cell inhibited by ARG were assessed by MTT and flow cytometry(FCM).Expression levels of Bcl-2 mRNA in SMMC-7721 cell were determined by RT-PCR.Results [...]
17
May
Effect of Silencing LRIG3 Gene on the Proliferation and Apoptosis of Bladder Cancer T24 Cells
This study examined the effect of silencing LRIG3 expression on the proliferation and apoptosis of bladder cancer T24 cells and explored the role of LRIG3 in the tumorigenesis of bladder cancer. Bladder cancer T24 cells were routinely cultured and pSilencer plasmids were employed to construct LRIG3 eukaryotic expression vector of LRIG3-siRNA, i.e., pSilencer-LRIG3-siRNA. After confirmation, [...]
17
May
Dracorhodin Perchlorate Suppresses Proliferation and Induces Apoptosis in Human Prostate Cancer Cell Line PC-3
The growth inhibition and pro-apoptosis effects of dracorhodin perchlorate on human prostate cancer PC-3 cell line were examined. After administration of 10-80 μmol/L dracorhodin perchlorate for 12-48 h, cell viability of PC-3 cells was measured by MTT colorimetry. Cell proliferation ability was detected by colony formation assay. Cellular apoptosis was inspected by acridine orange-ethidium bromide [...]
17
May
SKI-Ⅱ promoting apoptosis of SGC7901 cells
Objective:To study the effect and mechanism of sphingosine kinase inhibitor SKI-Ⅱ on the apoptosis of human gastric cancer cell SGC7901.Methods:SGC7901 cells were cultured and treated with SKI-Ⅱ alone or in combination with cisplatin(DDP).The apoptosis of the tumor cells was analyzed by flow cytometry;the morphological changes were observed by electron microscopy;the expression of Sphk1,NF-κB,Bcl-2 and Bax [...]
17
May
Mechanism of the apoptosis of human gastric cancer cell line SGC7901 induced by vascular endothelial growth factor siRNA
Objective The vascular endothelial growth factor(VEGF),a crucial promoter of blood vessel growth,is over-expressed in many types of tumor tissue.VEGF-targeted siRNA can effectively suppress the proliferation and induce the apoptosis of tumor cells.This study aims to investigate the mechanism of the apoptosis of human gastric cancer cell line SGC7901 induced by VEGF siRNA.Methods SGC7901 cells cultured [...]