In this studv by usine immunohistochemistry stain method Survivin and Caspase-3were detected in the colonic cancer tissue and normal tissue of 42patients,and colonic adenoma tissue of 38patients so as to investigate the expressions of the two proteins and their significance.As results,the positive expression rate of Survivin in the tissue of colonic cancer was significantly higher [...]
18
May
13
May
Potential signal pathway of resveratrol-induced apoptosis in human skin cancer cell line
Objective To investigate a potential signal molecule of resveratrol(Res)-induced apoptosis of human skin cancer cell line,A431 cells.Methods A431 cells were divided into 4 groups as group A(controls),group B(treated with Res),group C(pre-treated by SP600125) and group D(inhibitor control,SP600125 only).Phase contrast microscopy,Western blot and immunofluorescence staining were used to observe morphology and the expression of caspase-3,BCL-2,BAX,JNK and [...]
12
May
Dynamic Expression of Caveolin-1 and Caspase-3 Proteins in the Rat Lung Squamous Cell Cancer Formation
Objective: To investigate the dynamic expression caveolin-1(Cav-1) and Caspase-3 in relation to carcinogenesis,invasion,and metastasis in the rat lung squamous cell carcinoma.Methods: Squamous cell carcinoma of lung was induced with 3-methylcholanthrene in iodized oil by left intra-bronchial instillation in 80 Wistar rats.To obtain specimens in every pathological phase during the carcinogenesis,these rats were sacrificed at different [...]
11
May
Lanthanum chloride induces apoptosis in HeLa cells via Caspase-3 pathway
Objective To explore apoptosis-inducing effect of lanthanum chloride in cervical cancer cells in vitro and the related mechanism,so as to provide a theoretical basis for medical use of rare earth complex.Methods The anti-tumor activity of lanthanum chloride against HeLa cells was examined by morphological observation and MTT（3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide） assay.Distribution of cell cycle and cell apoptosis [...]
11
May
Effect of specific inhibition of survivin gene expression by small interfering RNA on the malignant behavior of human cervical cancer cell line Hela
Objective To study the effect of specific inhibition of survivin gene expression by small interfering RNA(siRNA) on the apoptosis of human cervical cancer cell line Hela.Methods The survivin siRNA was designed by web based software,synthesized and then transfected into hela cell line by lipofectamine 2000 reagent.The survivin gene expression inhibition and the expression of caspase-3 [...]
10
May
The role of P53 gene in radiation-induced death of non-small-cell lung cancer
Objective To investigate the role of P53 gene in radiation induced cell death of non-small-cell lung cancerMethods Select the H1299(p53-/-),H1299-P53,H1299-175H cells for the studing.Flow cytometry was used to detect the apoptosis rate,western bolt was used to detect the expression of related autophagy and apoptosis protein.Results When treated with 4 GY、8 GY H1299(P53-/-) manifest the lower [...]
9
May
9
May
NF-κB inhibitor-PDTC enhance TNF-α induced cytotoxicity in human cervical cancer HeLa cells and its mechanism
Objective To detect the effect of PDTC(the selective inhibitor of NF-κB) combined with TNF-α on the growth and apoptosis of human cervical cancer HeLa cells,and the related mechanisms.Methods After treated by different doses of PDTC,TNF-α or combined with the two on human cervical cancer HeLa cells,MTT assay was used to measure the growth inhibition of [...]
8
May
Expression and significance of NF-κBp65,c-IAP2,Caspase-3 in cervical cancer.
Objective:To study the expression and significance of nuclear transcription factor-κB(NF-κB)p65,Inhibitor of Apoptosis Protein(c-IAP2),Caspase-3(Caspase-3)in cervical carcinoma.Methods:NF-κBp65,c-IAP2 and Caspase-3 expressions were detected by immunohistochemistry in 44 cervical cancers,50 cervical intraepithelial neoplasia(CIN)and 30 normal cervical tissues.Results:The positive expression rate of NF-κBp65,c-IAP2 in cervical cancer and CIN were higher than in normal cervical tissue(P<0.05),while Caspase-3 was lower(P<0.05),the expression of [...]
6
May
Expression and significance of PUMA and caspase-3 in colorectal carcinoma
Objective: To observe the expression of PUMA and caspase-3 in colorectal cancer and adenoma,and study the significance of PUMA and caspase-3 in carcinogenesis and development of colorectal carcinoma.Methods: Immunohistochemical method was used to detect the expression of PUMA and caspase-3 in 44 cases of normal colorectal mucosa,20 cases of colorectal adenoma and 60 cases of [...]