Objective To study the inhibition effect of 5-Aza-2′deoxycytidine(5-Aza-CdR) on proliferation and apoptosis of gastric cancer cell line AGS and explore its possible mechanisms.Methods Low,median and high concentrations of 5-Aza-CdR were used to treat gastric cancer cell line AGS.Cell counting kit-8 was employed to detect the proliferation of gastric cancer cell line AGS;flow cytometer was used [...]
17
May
17
May
Effect and mechanism of β-elemene on apoptosis in human gastric cancer MGC803 cells
Objective To investigate the effect and mechanism of β-elemene on apoptosis in human gastric cancer MGC803 cells.Methods Human gastric cancer MGC803 cells were treated with different concentrations of β-elemene for 24～48 h,and the cell viability was measured by MTT assay,apoptosis was evaluated by flowcytometry following propidium iodide(PI) staining.Western blot was employed to detect the expression [...]
16
May
Anti-apoptotic role of HIF-1 in MCF-7 breast cancer cell line
HIF-1(hypoxia-inducible factor-1) is the major transcriptional factor responsible for oxygen-dependent gene expression.Suppression of HIF-1α is important for exploring HIF-1-dependent pathophysiological processes.In this study,we investigated the effect of short hairpin RNA(shRNA)targeting HIF-1α on the human breast carcinoma MCF-7 cell line.The protein level of HIF-1α increased after exposure of MCF-7 cells to hypoxia.However,apoptosis was lower in hypoxia [...]
16
May
Effect of Chinese herbal compound drug serum on the expressions of BID and CTNNB1 gene in human ovarian cancer cell line SKOV3
Objective:To study the effects of BID and CTNNB1 gene on apoptosis of human ovarian cancer cell line SKOV3 induced by Chinese herbal compound Lichongshengsui drink.Methods:Wistar rats were divided into two group at random and treated with pouring Lichongshengsui drink into stomach,blood samples were obtained to prepare Chinese herbal compound drug serum.mRNA of tumor cells in [...]
15
May
Effects on 5-Fluorouracil Induced Apoptosis and Cell Cycle Changes via Blocking Human Equilibrative Nucleoside Transporters in Pancreatic Cancer Cell Membrane
Objective To investigate the effects of dipyridamole(DP),one of the human equilibrative nucleoside transporters(hENTs) blockers,on 5-fluorouracil(5-FU) induced cell apoptosis and cell cycle changes of the pancreatic cancer Panc-1 cell line.Methods Pancreatic cancer cell line Panc-1 was divided into hENTs blocked group and hENTs unblocked group.The hENTs blocked group was further divided into two subgroups according to [...]
15
May
EFFECT OF RIBOFLAVIN ON THE MULTIPLICATIVE GROWTH OF ESOPHAGEAL CANCER CELL
[Objective]To investigate the effect of riboflavin on the multiplicative growth of esophageal cancer cell,and the relationship between riboflavin and esophageal cancer.[Methods]The human esophageal cancer cell line Eca109 was cultured.(1)We used different concentration of riboflavin for Eca109,and we used MTT colorimetry in termination of the multiplicative growth of Eca109,and calculated the proliferation rate of cells.(2)We determinated [...]
14
May
Expression of survivin and CyclinD1 genes in Hazak’s esophageal squamous cell carcinoma
Objective To study the expression of CyclinD1 and survivin genes in Hazak′s esophageal cancer.Methods The expression of CyclinD1 and survivin genes were detected in 30 cases of esophageal cancer tissues and non-esophageal cancer tissues by reverse transcription polymerase chain reaction.Results The positive rates of CyclinD1 gene expression in tissues of esophageal squamous cell carcinoma,dysplasia and [...]
13
May
Experimental Research on the Extracts of Viscum Coloratum for Inhibiting the Proliferation and Induced Apoptosis of HepG2 Cells of Human Liver Cancer
Objective:To explore the anti-proliferation and apoptosis-induced effects of Viscum coloratum extracts(VCE) on HepG2 cells.Methods:HepG2 cell proliferative activity was measured by MTT assay.Cell apoptosis was observed by Giemsa staining method.Cell apoptosis rate was determined by flow cytometry.Results:After exposure to VCE at concentrations 0.3mg·mL-1 for 24h,cellular proliferation was inhibited with the increase of the concentration and the [...]
12
May
Cathepsin B and Paclitaxel-Induced Apoptosis in Gastric Cancer BGC-823 Cells
Objective To study the relation between cathepsin B and paclitaxel-induced apoptosis in BGC-823 cells(gastric cancer cells).Methods BGC-823 cells density was adjusted for 1×105/holes,one group cells were treated with paclitaxel of various concentration(0-0.5 μmol/L) for 48 h and the other group were treated with 0.3 μmol/L paclitaxel of various time(6-48 h).The above paclitaxel exposure group each [...]
11
May
Induction of Apoptosis in Malignant Melanoma Cells by LDE and its Mechanisms
Objective To study the effect of action of LD extract(LDE)on the growth of malignant melanoma cells and its mechanisms.Methods The effects of LDE on the growth of the melanoma tumor cell line B16 was evaluated by MTT.The LDE-induced apoptosis of the cancer cells was detected by labeling with fluorescence probe.The apoptotic morphological changes were observed [...]