Objective To observe the effects of Pak1 siRNA to cell growth and cell cycle of gastric cancer cell BGC823.Methods The expression of Pak1 in gastric cancer cell was detected by Western blot.Pak1 siRNA was transfected into BGC823 cell.The growth ability of BGC823 cell was observed by MTT assay and counting cell number,and the cell cycle [...]
18
May
17
May
Progress of Study on the Relationship Between the Spindle Checkpoint and Carcinoma
The spindle checkpoint is an important cell-cycle surveillance mechanism that ensures the fidelity of chromosome segregation during mitosis and is required for genetic stability.It has become increasingly clear that malfunction of the cell-cycle surveillance mechanism involving the spindle checkpoint contributes to cancer formation.Accumulated evidence shows that deregulated expression,genotypic polymorphism or promoter methylation of the mitotic [...]
17
May
Inhibition Effects of Anti-cancer Bioactive Peptide on Hepatocarcinoma H22 Tumor-bearing Mice
Objective:To investigate the effects of anti-cancer bioactive peptide(ACBP) on hepatocarcinoma H22 tumor-bearing mice,and to elucidate the possible mechanisms of inhibition.Methods: Animal model of hepatocarcinoma H22 tumor-bearing mice were established.And the mice were randomly divided into three groups,and were treated with 5-Fu,ACBP,physiological saline by caudal vein injection,respectively.The mice were fed 10 days and were treated every [...]
16
May
The role of BRCA1 in DNA damage repair
BRCA1 is a well-established tumor suppressor gene,which is frequently mutated in familial breast and ovarian cancers.The function of BRCA1 in a number of cellular pathways is to maintain genomic stability,including DNA damage-induced cell cycle checkpoint activation,DNA damage repair,transcriptional regulation,apoptosis and ubiquitination.In this review,we discuss recent advances regarding our understanding of the role of BRCA1 in [...]
16
May
15
May
Establishment of gastric cancer cell line with stable over-expression of Cdx2
Objective To establish a gastric cancer cell line with stable over-expression of Cdx2 gene.Methods The recombined eukaryotic expression vector pCMV-Cdx2-HA or empty vector pCMV-HA was transfected into gastric cancer MGC-803 cells by Lipofectamine.G418-resistant colonies were selected by adding G418 to the medium.Cdx2 mRNA and protein expression was assessed by RT-PCR and Western blot.The cell cycle [...]
15
May
Effects on 5-Fluorouracil Induced Apoptosis and Cell Cycle Changes via Blocking Human Equilibrative Nucleoside Transporters in Pancreatic Cancer Cell Membrane
Objective To investigate the effects of dipyridamole(DP),one of the human equilibrative nucleoside transporters(hENTs) blockers,on 5-fluorouracil(5-FU) induced cell apoptosis and cell cycle changes of the pancreatic cancer Panc-1 cell line.Methods Pancreatic cancer cell line Panc-1 was divided into hENTs blocked group and hENTs unblocked group.The hENTs blocked group was further divided into two subgroups according to [...]
15
May
EFFECT OF RIBOFLAVIN ON THE MULTIPLICATIVE GROWTH OF ESOPHAGEAL CANCER CELL
[Objective]To investigate the effect of riboflavin on the multiplicative growth of esophageal cancer cell,and the relationship between riboflavin and esophageal cancer.[Methods]The human esophageal cancer cell line Eca109 was cultured.(1)We used different concentration of riboflavin for Eca109,and we used MTT colorimetry in termination of the multiplicative growth of Eca109,and calculated the proliferation rate of cells.(2)We determinated [...]
15
May
Effects of deleted in liver cancer 1 gene transfection on the proliferation and migration of human ovarian cancer cell line OVCAR-3
Objective:To investigate the effects of deleted in liver cancer 1(DLC1) gene on the proliferation and migration of human ovarian cancer cell line OVCAR-3.Methods:The recombinant plasmid pEGFP-C3-DLC1 was transfected into OVCAR-3 cells by lipofectAMINE.The expressions of DLC1 mRNA and protein before and after transfection were detected by reverse transcriptase-polymerase chain reaction(RT-PCR) and Western blotting,respectively.The proliferation,cell cycle [...]
15
May
Targeting the cell cycle in esophageal adenocarcinoma:An adjunct to anticancer treatment
Esophageal adenocarcinoma is a major cause of cancer death in men in the developed world.Continuing poor outcomes with conventional therapies that predominantly target apoptosis pathways have lead to increasing interest in treatments that target the cell cycle.A large international effort has led to the development of a large number of inhibitors,which target cell cycle kinases,including [...]