Objective To investigate the multiple molecular targeted agent,sorafenib,in human gastric cancer SGC-7901 cell proliferation,apoptosis and P-ERK expression,and explore its possible mechanism.Methods MTT method was used to detect antiproliferative ratio of sorafenib on human gastric cancer SGC-7901 cell;immunocytochemical method for detection of gastric cancer cells P-ERK protein expression;and flow cytometry to analyze gastric cancer cell apoptosis.Results [...]
17
May
17
May
Effect of Silencing LRIG3 Gene on the Proliferation and Apoptosis of Bladder Cancer T24 Cells
This study examined the effect of silencing LRIG3 expression on the proliferation and apoptosis of bladder cancer T24 cells and explored the role of LRIG3 in the tumorigenesis of bladder cancer. Bladder cancer T24 cells were routinely cultured and pSilencer plasmids were employed to construct LRIG3 eukaryotic expression vector of LRIG3-siRNA, i.e., pSilencer-LRIG3-siRNA. After confirmation, [...]
17
May
Effects of lanthanum chloride on the proliferation and tumor-associated miRNAs’ expression in human colorectal cancer HT-29 cells
Objective To investigate the effects of lanthanum chloride on the proliferation of human colorectal cancer HT-29 cells and the expression of tumor-associated microRNAs.Methods Human colorectal cancer HT-29 cells were cultured routinely in vitro.The inhibition effects of lanthanum chloride on cell-proliferation were determined by MTT method.The effects of lanthanum chloride on the expression of microRNA let-7 [...]
17
May
Effect of YKL-40 Transfection on Proliferation and Invasion Activities of Prostate Cancer LNcap Cells
Objective To investigate exogenous YKL-40 gene transfection on the proliferation,invasion,migration and adhesion activities of prostate cancer LNcap cells.Methods The expressions of endogenous YKL-40 gene in prostate cancer DU-145,PC-3 and LNcap cells were determined by RT-PCR.LNcap cells were transfected with plasmid pcDNA3.1-YKL-40 and determined for proliferation,invasion,migration and adhesion activities before and after transfection by MTT method,Boyden [...]
16
May
STAT2 shRNA Inhibits HeLa Cell Proliferation
Recently it was reported that signal transducer and activator of transcription 2(STAT2) involves in carcinogenesis. Previous study showed that several kinds of tumor tissues as well as tumor cell lines highly expressed STAT2. Therefore,in order to further explore the effects of STAT2 on carcinogenesis,STAT2 expression level in cervical cancer cell line HeLa was decreased by [...]
15
May
Effects of deleted in liver cancer 1 gene transfection on the proliferation and migration of human ovarian cancer cell line OVCAR-3
Objective:To investigate the effects of deleted in liver cancer 1(DLC1) gene on the proliferation and migration of human ovarian cancer cell line OVCAR-3.Methods:The recombinant plasmid pEGFP-C3-DLC1 was transfected into OVCAR-3 cells by lipofectAMINE.The expressions of DLC1 mRNA and protein before and after transfection were detected by reverse transcriptase-polymerase chain reaction(RT-PCR) and Western blotting,respectively.The proliferation,cell cycle [...]
14
May
Inhibitory effect of ginsenoside Rg1 on BGC-823 human gastric cancer cell line
AIM: To observe the influences of Ginsenoside Rg1 on the activity,proliferation,the expression of Bax-2 and caspase-3 and morphology of BGC-823 gastric cancer cell line,and investigate its pharmacological mechanisms. METHODS: Cells which were stayed in logarithmic growth were used for the experiments,the cells were interfered with different concentration of Ginsenoside Rg1,the methods of growth curve,M’IT assay,protein [...]
14
May
Over-expression of STC1 gene suppresses the proliferation of human cervical cancer cell line CaSki
Objective To observe the influences of STC1 gene on the proliferation and cell cycle of human cervical cancer cell line CaSki. Method CaSki cells were divided into three groups: the experimental group transfected with plasmid pcDNA3.1(+)-STC1;Empty vector group transfected with pcDNA3.1(+);blank control group transfected without any interference. The stable expression clones were chosen and the [...]
14
May
Effects of Genistein on biological behavior of human hepatocarcinoma cells in vitro
【Objective】 To explore the effect of Genistein(Gen) on the biological behaviors including proliferation,adhesion,migration and invasion capabilities of hepatocellular carcinoma SMMC7721 cells in vitro.【Methods】 SMMC7721 cells were treated with Genistein at different concentrations for various time.Cell proliferation was assessed using MTT assay.The migration and invasion capabilities of SMMC7721 cells were determined by Transwell method(Boyden chamber).Expression levels [...]
11
May
Effects of deguelin on proliferation and apoptosis of MCF-7 breast cancer cells by phosphatidylinositol 3-kinase/Akt signaling pathway
Objective:To study the effects of deguelin on proliferation and apoptosis of human breast cancer cell line MCF-7 and on phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway.Methods:After treatment with 0,1,5,10,15 and 20 μmol/L of deguelin for 6,24,48 and 72 hours,the proliferation inhibition rate of MCF-7 cells was measured by cell counting kit-8 assay. Apoptosis rate of [...]