Objective To investigate the effects of NDRG1 on proliferation,invasion and migration of human colorectal cancer cell line HT-29 in vitro.Methods LipofectamineTM 2000 was used to transfect NDRG1 into human colorectal cancer HT-29 cells.The expression level of NDRG1 was measured by fluorescence microscopy and immunocytochemical staining.The cell cycle distribution was determined by fluorescence-activated cell sorting(FACS).The ability [...]
18
May
18
May
Expressions of DLK1 and ABCG2 in primary human hepatocellular carcinoma
Objective To investigate the expressions of DLK1(delta drosophila homolog-like1) and ABCG2(ATP-binding cassette superfamily G member 2)in primary human hepatocellular carcinoma(HCC).Methods The expressions of DLK1 protein and ABCG2 protein in 150 samples of HCC,50 samples of cirrhosis,50 samples of hepatitis and 10 samples of normal liver tissues were assessed by immunohistochemistry.The expressions of DLK1 mRNA and [...]
16
May
Relationship of KAI-1 and nm23-H1 Expressions to Invasion and Metastasis of Colorectal Cancer
Objective To explore the relationship of KAI-1 and nm23-H1 expressions to the invasion and metastasis of colorectal carcinoma,to find their role in the invasion and metastasis.Methods The mRNA and protein levels of KAI-1 and nm23-H1 were determined by fluorescent real-time quantitative PCR and immunohistochemical methods in 45 colorectal carcinoma specimens.Results The mRNA and protein expressions [...]
16
May
Prokaryotic Expression and Purification of Minor Capsid Protein L2 Derived from Human Papillomavirus 58 and Preparation of Monoclonal Antibody
Objective To express the minor capsid protein L2 derived from human papillomavirus(HPV) 58 which caused majority of cervical cancer in China in prokaryotic cells,purify the expressed product and prepare its monoclonal antibody(McAb).Methods L2 gene was amplified from plasmid pHPV58 containing the genome of HPV-58 and inserted into modified prokaryotic expression vector pGEX-KGV.The constructed recombinant plasmid [...]
16
May
Prokaryotic Expression and Purification of Minor Capsid Protein L2 Derived from Human Papillomavirus 58 and Preparation of Monoclonal Antibody
Objective To express the minor capsid protein L2 derived from human papillomavirus(HPV) 58 which caused majority of cervical cancer in China in prokaryotic cells,purify the expressed product and prepare its monoclonal antibody(McAb).Methods L2 gene was amplified from plasmid pHPV58 containing the genome of HPV-58 and inserted into modified prokaryotic expression vector pGEX-KGV.The constructed recombinant plasmid [...]
16
May
Expression of Plk1 and Survivin in hepatocellular carcinoma and its significance
Objective Investigating expression of Plk1 and survivin in hepatocellular carcinoma.providing theory for diagnosis and therapy in clinic.Methods Immunohistochemical analysis was used to detect the expression of Plk1 and Survivin in 100 cases of hepatocellular carcinoma.Results â‘´The positive rate of survivin expression in HCC was significantly higher than that in normol liver tissues(P<0.05).There was no correlation [...]
16
May
Associated expression of Plkl and p21WAF1/CIP1 in hepatocellular carcinoma
Objective It has been shown that the cell cycle inhibitor p21WAF1/CIP1(p21)can lead to the transcriptional inhibition of the Polo-like kinase 1(Plk1) gene,this study was aimed to investigate the expression of Plk1 and p21 genes in hepatocellular carcinoma(HCC) and to explore the expression correlations between the two genes.Methods Expression of Plkl and p21 protein was evaluated [...]
14
May
Screening of high and low expressed RSK4 genehuman breast cancer cell lines
OBJECTIVE:To detect the RSK4 gene expression in MDA-MB-231,T47D,Bcap37,MCF-7 human breast cancer cell lines,and select the highest and lowest expression of RSK4 cell lines for the further study.METHODS:RT-PCR and Western-blot were used to verify the mRNA and protein levels of expression of RSK4.RESULTS:RSK4 gene expressed in all four human breast cancer cell lines,and the expression of [...]
14
May
Study on correlation of HPV16/18 E6 infection and FHIT expression in cervical cancer
Objective To determine the expression of HPV16/18 E6 and FHIT protein in cervical cancer,and the relationship between their expressions.Methods Immunohistochemistry was used to detect the expression of HPV16/18 E6 and FHIT protein in 10 normal cervices as well as 40 cervical cancers.Results The expression of HPV16/18 E6 protein in cervical cancer was much higher than [...]
14
May
Experimental study on factor of tumor-related gene expression in tumor tissue,adjacent proximal and distal of MKN-45 human gastric cancer orthotopic transplanta
Objective After treatment of gastric cancer such as surgery,tumor is easy to recurrence or metastasis,detect tumor-associated factor expression in tumor tissue,proximal tissue away from tumors and distal tissue away from tumors to clear the importance of improving the residual stomach or body microenvironment of the tumor-prone.Methods After orthotopic,take MKN-45 gastric cancer tissues,proximal tissue away from [...]