Objective:To investigate the joint therapeutic effect of Liuwei Dihuangwan(LD) combined with hepatocarcinoma HSV-tk/GCV suicide gene on the expression of connexin(Cx),clarity the therapeutic mechanism of LD.Method:Ninety Kunming mice were randomized into 4 groups:model group,LD group(10 g·kg-1·d-1),suicide gene therapy group(intraperitoneal injection of guanosine ganciclovior) and the combined group.The transplanted tumor mice model of H22 hepatocarcinoma was established [...]
15
May
15
May
Influence of MACC1 Gene expression on the proliferation and migration of SBC-5 lung cancer cells
Objective:To investigate the influence of siRNA targeting metastasis-associated in colon cancer l(MACC1) gene on proliferation and migration of human lung cancer cell line SBC-5 in vitro.Methods:The siRNA targeting MACC1 gene and negative control siRNA were chemically synthesized,and were then transiently transfected into human lung cancer cell line SBC-5 by LipofectamineTM 2000.Expression of MACC1 mRNA and [...]
14
May
The growth inhibitory effects by transfection of p16 gene on human hepatic cancer cell line*
Objectiv p16 gene is a tumor suppressor gene and is inactivated in the carcinogenesis of hepatic cancer. This paper was to test ability to inhibit growth of hepatic cancer cell of p16 gene. Method p16 cDNA was subcloned to pcDNA3.1 eucaryotic expressing vector; then the recombinant pcDNA3.1-p16 plasmi plasmid was transfected into human hepatic cell [...]
12
May
Efficient inhibition of murine Lewis lung cancer by Survivin T34A plasmid/liposome complexes
Objective This study was to explore the effects and mechanism of Survivin mutant Thr34→Ala(Survivin T34A)on Lewis lung cancer.Methods Plasmid encoding the phosphorylation defective dominant-negative mouse survivin threonine 34→alanine mutant(Survivin T34A)complexed to a DOTAP-chol liposome(Lip-mS)and a empty plasmid complexed to a DOTAP-chol liposome(Lip-null)were administered in Lewis Lung Carcinoma(LLC)cells and in mice bearing LLC tumors separately,compared with [...]
11
May
Construction and identification TRF2 siRNA expression vector on the breast cancer MCF-7 cells
Objective:To construction adenovirus-mediated RNA interference vector expressing TRF2 small interfering RNA(rAd-shRNA-TRF2),and explore the specific inhibitory effect of this vector on TRF2 mRNA expression in MCF-7 cell.Methods:The recombinant adenovirus rAd-shRNA-TRF2 was obtained,and the vector was transfected into MCF-7 cells,then the expression of TRF2 mRNA and protein at 48 h after transfection was detected by FQ-RT-PCR and [...]
9
May
Studies on adenovirus-mediated HSV-tk/GCV gene therapy controlled by hTERT promoter for human bladder cancer
Objective To explore the selective killing effect of adenovirus-mediated simplex thymidine kinase(HSV-tk)/GCV gene therapy controlled by human telomerase reverse transcriptase(hTERT) promoter on human bladder cancer in vitro and in vivo.Methods Ad-hTERT-HSV/tk and Ad-CMV-HSV/tk were transfected into 253J and MRC-5 followed by GCV treatment.The relative survival rate of cells in presence of prodrug GCV was measured [...]
8
May
7
May
Effects of PRL-3 gene on the proliferation and apoptosis of breast cancer MCF-7 cells
Objective:To study the effects of short hairpin RNA(shRNA) targeting PRL-3 gene on the proliferation and apoptosis of breast cancer MCF-7 cells.Methods:The PRL-3 specific shRNA expression vector was constructed and confirmed by sequencing analysis.PRL-3-shRNA expression vector was transfected into MCF-7 cells via lipofectamineTM 2000,and the expression levels of PRL-3 mRNA and protein compared with those non-transfected [...]
5
May
Anti-sense nucleic acid of CyclinD1 induces apoptosis of lung adenocarcinoma cancer cell A549
To explore the potential of the anti-sense nucleic acid of CyclinD1 in lung cancer therapy,the expression vector containing the anti-sense nucleic acid of CyclinD1 was constructed and named pcDNA3.1-CyclinD1.The A549 cells were transfected with pcDNA3.1-CyclinD1 vectors.After being screened by G418,the stable expression positive clones were obtained.MTT method and flow cytometry technique were used to detect [...]
5
May
Recombinant human PTEN suppresses gastric carcinoma cell growth in vitro
Objective:To study the effect of Ad-PTEN gene in SGC-7901tumor cells.To study the inhibition and potential molecular mechanisms of adenovirus-mediated expression of Ad-PTEN gene in SGC-7901 gastric cancer cells.Methods: PTEN gene was transfected into human gastric cancer cell line SGC-7901 with a replication-incompetent adenovirus vector.Using RT-PCR to detect the mRNA expression of PTEN.Using light microscope and [...]