Objective: To isolate stem-like cells from hepatocellular carcinoma cell line PLC/PRF-5 and to study their miRNA profile.Methods:ABCG2+ and ABCG2-PLC/PRF-5 cells were isolated from the PLC/PRF-5 cell line by magnetic activated cell sorting(MACS) method,and further identified by flow cytometry.The colony formation ability in soft agar and tumor formation ability in NOD/SCID mice of ABCG2+ and ABCG2-PLC/PRF-5 [...]
18
May
16
May
ANXA-2 and VEGF promote invasiveness of human liver cancer HepG2 cells
AIM:To investigate the role of annexin-2(ANXA-2)and vascular endothelial growth factor(VEGF)in promoting invasiveness of human liver cancer HepG2 cells.METHODS:After HepG2 cells were treated with different doses of 5-fluorouracil,cell invasiveness was detected by Transwell assay,and the mRNA and protein expression of ANXA-2 and VEGF was detected by RT-PCR and Western blot,respectively.RESULTS:The invasiveness of HepG2 cells decreased with [...]
16
May
Clinical application of liver cancer resection combined with splenic microwave ablation
Objective To investigate the clinical value of liver cancer resection combined with splenic microwave ablation for treating the patients with hepatocellular cancer and hypersplenism.Methods From August 2009 to January 2010,the clinical data of five cases with hepatocellular cancer associated with hypersplenism received liver cancer resection combined with splenic microwave ablation were analyzed retrospectively.The changes of [...]
15
May
Effect of three dimensional conformal radiation therapy combined with thalidomide on advanced hepatic carcinoma
Objective: To observe the efficacy and safety of three dimensional conformal radiation therapy(3-DCRT) combined with thalidomide(TLD) on advanced hepatic carcinoma.Methods: Forty-four patients with primary liver cancer were randomly divided into therapy group and control group.The TLD group were treated by 3-DCRT combined with TLD,and the control group were only treated by 3-DCRT.The main outcome measure [...]
15
May
MRP in typing and superiority to the hepatocellular carcinoma with portal vein tumor thrombosis
Objecttive To explore the aplication of MRP in typing and superriority to the hepatocellular carcinoma with portal vein tumor thrombosis.Mothods 126 patients data with primary hepatocellular carcinoma were collected from March,2006 to December,2009 after the MRP imaging formation inspection,of those 84 examples with concurrent portal vein cancer embolism.Typing determination were made for portal vein tumor [...]
15
May
Recent advances in liver cancer stem cell research
Over the past few years,compelling evidence has emerged in support of the cancer stem cell(CSC) model for many solid tumors including hepatocellular cancers.The CSCs are posited to be responsible not only for tumor initiation but also for the generation of distant metastasis and relapse after therapy.Therefore,identification and detailed characterization of liver CSCs is imperative for [...]
15
May
CT guided percutaneous Argon-Helium cryoablation on quality of life of huge hepatocellular carcinoma older patients
Objective To observe the changes of quality of life(QOL) of huge hepatocellular carcinoma(HCC) patients over 60 years treated with CT guided percutaneous Argon-Helium cryoablation(PAHC).Methods There were 91 HCC patients over 60 years who had received CT guided PAHC.The diameter of tumors in all the patients was larger than 10 cm.The qualities of life of the [...]
13
May
Expression of EGF and c-myc in hepatocellular carcinoma tissues and its clinical implications
Objective To study expression of EGF and c-myc in patients with hepatocellular carcinoma(HCC).Methods EGF and c-myc proteins were examined using tissue microarray and in conjunction with immunohistochemistry for 60 patients with hepatocellular carcinoma.A control of 15 normal tissue samples(20 mm away from the cancers) was also included.Results Expression rates of EGF and c-myc were significantly [...]
13
May
Effect of mammalian target of rapamycin on side population cells in hepatocellular carcinoma cell line Hep3B
Objective To investigate the effect of mammalian target of rapamycin(mTOR) on the side population(SP) cells in hepatocellular carcinoma(HCC) cell line Hep3B cells.Methods Rapamycin inhibited mTOR,the proportion of SP cell was analyzed by flow cytometry.The clone formation SP cells and non-SP cells in Hep3B cells was detected by soft agar clone formation methoad.Results There were SP [...]
12
May
MAGI1 inhibits cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN
Objective To explore the biological function and molecular mechanism of membrane associated guanylate kinase,WW and PDZ domain containing 1(MAGI1) in hepatocellular carcinoma.Methods HepG2MAGI1 stable cell line was constructed by transfecting HepG2 cells with pcDNA3.1-MAGI1 plasmid.Wound healing and invasion assay were performed to compare the migration and invasion ability of HepG2MAGI1 and HepG2 cells.Furthermore,the expression of [...]