Objective To construct an eukaryotic expression vector encoding decorin(DCN)(pEGFP-N1-DCN) and determine its effect on proliferation,cell cycle,migration and invasion of human gastric cancer cell line SGC-7901 after transfection.Methods SGC-7901 cells were divided into 3 groups,control,pEGFP-N1 group and pEGFP-N1-DCN group.SGC-7901 cells were transfected by corresponding plasmids with liposome.The expressions of epidermal growth factor receptor(EGFR) and transforming growth [...]
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May
Cisplatin-resistance increases proliferation,invasion and migration of ovarian cancer OVCAR-3 cells
Objective: To investigate the differences in biological behavior between cisplatin-sensitive(OVCAR-3 cells) and cisplatin-resistant human ovarian cancer cells(OVCAR-3/CDDP cells),and to explore the possible mechanisms.Methods: OVCAR-3 and OVCAR-3/CDDP cells in logarithmic phase were collected,their proliferation were assessed by soft agar colony formation assay,their in vitro and in vivo invasion and migration abilities were measured by Transwell chamber [...]
16
May
Effects of Src on Proliferation and Invasion of Lung Cancer Cells
Background and objective It has been proven that Src played pivotal roles in carcinogenesis,cancer progression and metastasis. The aim of this study is to explore the roles of Src phosphorylation on lung cancer cells. Methods Western blot and immunoprecipitation was used to detect the expression and phosphorylation of Src in lung cancer cells. MTT and [...]
15
May
Correlation between expression of Cyclin E in primary breast cancer cells and Invasion of breast cancer cells
Objective:To study the Correlation between expression of Cyclin E in breast cancer and invasion of primary breast cancer cells.Methods:1cm3 of the breast cancer tissue was taken got from operation,prepareing breast cancer cells suspension of origin generation,and detecting the expression level of Cyclin E by immunofluorescent technique.These cancer cells of origin generation were detected for invasion [...]
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May