Objective To study the situation of DAPK1 gene methylation and the expression of DAPK1 in human gatric cancer tissues.Methods The methylation of DAPK1 was detected by MSP,and the expression of DAPK1 was detected by RT-PCR.Results The low expression of DAPK1′s mRNA is 16/35(P=0.465).The rate of DAPK1 gene methylation in human gastric cancer tissues and cancer-adjacent [...]
18
May
18
May
Effect of CHFR CpG island methylation status on proliferation and apoptosis of esophageal cancer Eca109 cells
Objective:To study the relationship of CHFR CpG island methylation status on proliferation and apoptosis of esophageal cancer Eca109 cells.Methods: Eca109 cells were treated with different concentrations(2,5,and 10 μmol/L) of 5-Aza-2′-deoxycytidine(5-Aza-CdR),and untreated Eca109 cells were used as control.Methylation-specific PCR(MSP) analysis was used to evaluate the CpG island methylation status of CHFR gene,and RT-PCR was used to [...]
17
May
Cancer systems biology:signal processing for cancer research
In this editorial we introduce the research paradigms of signal processing in the era of systems biology.Signal processing is a field of science traditionally focused on modeling electronic and communications systems,but recently it has turned to biological applications with astounding results.The essence of signal processing is to describe the natural world by mathematical models and [...]
17
May
Tumor-associated methylation of the putative tumor suppressor AJAP1 gene and association between decreased AJAP1 expression and shorter survival in patients wit
Allelic loss of the short arm of chromosome 1 has been observed frequently in a wide spectrum of cancers,most frequently in oligodendroglioma.In our previous studies,we evaluated 177 oligodendroglial tumor samples and identified the AJAP1 gene(formerly Shrew1) in the consensus region of deletion.AJAP1 is a transmembrane protein found in adheren junctions and functions to inhibit glioma [...]
17
May
Inhibition effect and mechanism of 5-Aza-CdR on proliferation and apoptosis in gastric cancer cell line AGS
Objective To study the inhibition effect of 5-Aza-2′deoxycytidine(5-Aza-CdR) on proliferation and apoptosis of gastric cancer cell line AGS and explore its possible mechanisms.Methods Low,median and high concentrations of 5-Aza-CdR were used to treat gastric cancer cell line AGS.Cell counting kit-8 was employed to detect the proliferation of gastric cancer cell line AGS;flow cytometer was used [...]
17
May
Correlation of Cyclin A1 Gene Hypermethylation in Promoter Region and Metastasis of Breast Carcinoma
Objective:To investigate the methylation pattern of CCNA1 in breast carcinoma and explore the role of these epige-netic changes in tumorgenensis.Methods:95 specimens of breast cancer were used,and the DNA was extracted by Qiagen procedure.Methylation change in the promoter region of CCNA1 was determined by using restricted enzyme cut-PCR.Results:The positive rate of gene promoter hypermethylation of CCNA1 [...]
17
May
Experimental studies of 5-Aza-CdR on p27kip1 gene’s abnormal methylation in gastric cancer cell line
Objective To study the effect of 5-Aza-CdR on p27kip1 gene’s abnormal methylation in gastric cancer cell line.Methods The cells were treated with different concentrations of 5-Aza-CdR.The methylation of p27kip1 in the cell line was detected by MSP,and the expression of p27kip1 was detected by RT-PCR and westernblot.Results The methylation of p27kip1 cells were changed with [...]
14
May
Relationship between P16INK4a Methylation and Cervical Cancer
P16INK4a gene is the first known tumor suppressor gene involved in cell cycle regulation.The abnormal methylation of P16INK4a is an early event in the process of cervical cancer,and the methylation rate increases with the development of cervical cancer.The abnormal methylation of P16INK4a may be a new tumor marker in clinical screening and diagnosis of cervical [...]
12
May
Real-time Fluorescence Quantitative PCR Method for Determination of the TWIST1 Gene Promoter Methylation Status in Bladder Cancer
Objective To establish the quantitative detection methods for TWIST1 gene methylation status in order to assess its clinical research in bladder cancer application.Methods Methylation status and unmethylation status of TWIST1 promoter were detected by realtime quantitative PCR in 50 cases of bladder cancer patients in Nantong and 25 cases of non-bladder cancer urine samples,the frequency [...]
10
May
Effect of 5-Aza-CdR on Expression of T-cadherin Gene and Cell Proliferation in Human Esophageal Carcinoma Cell Line
Background:T-cadherin may function as a tumor suppressor gene in the process of tumorigenesis,and down-regulation of T-cadherin in various human cancers has been reported,including esophageal carcinoma.Aims:To investigate the effect of demethylating agent 5-azacytidine-2′-deoxycytidines(5-Aza-CdR)on expression of T-cadherin gene and cell proliferation in human esophageal carcinoma cell line EC109.Methods:Human esophageal carcinoma cell line EC109 was cultured conventionally and [...]