Objective:To develop a 5-fluorouracil(5-Fu)-resistant human pancreatic cancer cell line Patu8988/5-Fu and investigate its biological characteristics and the preliminary mechanisms of chemoresistance in pancreatic cancer.Methods:Patu8988 was exposured to increased 5-Fu progressively for 10 months to obtain stable 5-Fu-resistant Patu8988/5-Fu in vitro.The morphological features and growth characters of Patu8988/5-Fu were observed.MTT assay was used to observe its [...]
17
May
17
May
Effect of different heating methods combined with neferine on the expressions of γH2AX and mdr-1/P-gp in MCF-7/Adr breast cancer cells
Objective To determine the effect of different heating methods combined with neferine(Nef) on the proliferation and expressions of γH2AX and mdr-1/P-gp in MCF-7/Adr breast cancer cells.Methods MTT assay was used to determine block heating,water submerged heating,medium heating,and oven heating combined with 10 μg/mL Nef on adriamycin cultured MCF-7/Adr cell proliferation.The mdr-1mRNA expression was detected by [...]
17
May
Zosuquidar reverses P-glycoprotein-mediated multidrug resistance in patients with liver cancer
Objective To reverse multidrug resistance(MDR) by zosuquidar trihydrochloride(LY335979,zosuquidar.3HC1) in the patients with liver cancer.Methods 99mTc-MIBI single-photon emission computed tomography(SPECT) was performed at 15 min and 120 min after injection of 20 mCi 99mTc-MIBI,and early uptake,delayed uptake(L/Nd) and washout rate(L/Nwr) of 99mTc-MIBI were obtained in 60 patients with liver cancer.99mTc-MIBI SPECT examination was repeated after oral [...]
16
May
Effect of Tetrandrine to Multidrug Resistance and Apoptosis of Pancreatic Cancer Cells
Objective To explore the effect of Tetrandrine to the multidrug resistance and apoptosis of pancreatic cancer cell.Methods Human pancreatic cancer cells BxPC-3 sensitive to Gemcitabine and BxPC-3/Gem resistant to Gemcitabine were selected and divided into 3 groups,the control group、Gemcitabine group and Gemcitabine with Tetrandrine group.The cytotoxic effect of anticancer drugs was examined by MTT methods [...]
15
May
Investigation of multidrug-resistant related genes of human SCLC using cDNA microarray
Objective To screen the small cell lung cancer(SCLC) resistance-related genes,providing ideal targets for sensitizing the multidrug-resistant SCLC to chemotherapy.Methods Overexpressed genes in multidrug-resistant SCLC were selected according to the cDNA microarray.QRT-PCR was applied for assessment of the expression of targeted genes in 6 patients with SCLC before chemotherapy and at the presentation of drug resistance.Results [...]
14
May
Role of epithelial-mesenchymal transition in drug resistance of human colon cancer cell line SW480 to cetuximab and 5-fluorouracil
Objective To discuss the epithelial-mesenchymal transition(EMT) of human colon cancer cell line SW480 and its effect on tumor drug resistance to chemotherapy drugs and targeted drugs.Methods SW480 cells were treated with cetuximab(C225),5-fluorouracil(5-FU),and C225 and 5-Fu combination,separately.Cell morphology was observed.Immunofluorescence assay was employed to detect the cytoskeleton changes as well as the expression difference of E-cadherin [...]
12
May
Multidrug resistance-associated proteins and their roles in multidrug resistance
Multidrug resistance(MDR) is a major impediment to the success of cancer chemotherapy.Multidrug resistance-associated proteins(MRPs) play an important role in the process of MDR.As an ATP-binding cassette(ABC) transporter superfamily,MRPs are selective and specific drug efflux pumps.In this paper,physiological characteristics,structural characteristics and resistance profile of MRPs and the associated reversal studies are reviewed.
12
May
Neoadjuvant endocrine therapy in MDR protein positive locally advanced breast cancer
Objective To evaluate the clinical efficacy of neoadjuvant endocrine therapy(NET) for multidrug resistant(MDR) proteins positive breast cancer.Methods Sixty-two postmenopausal women with locally advanced breast cancer(LABC) and estrogen receptor(ER) and MDR protein positive were randomly devided into two groups: Neoadjuvant chemotherapy(NCT) group,receiving 4 courses of TEC(taxotere,epirubicin and cyclophosphamide) chemotherapy regimen,and NET group,receiving 3 months of letrozole [...]
8
May
Intratumoral injection of ricin-loaded thermosensitive hydrogel for the treatment of paclitaxel resistant ovarian cancer xenograft
Objective To investigate the effectiveness of ricin-loaded thermosensitive hydrogel for intratumoral injection in treatment of paclitaxel resistant ovarian cancer xenograft.Methods The paclitaxel resistant ovarian cancer cell line A2780/PTX was established with gradient concentration increase method.The mice with A2780/PTX tumor were divided into 6 groups: model group,gel bank group,paclitaxel group,paclitaxel gel group,ricin toxin group and ricin-gel [...]
7
May
Effect of miR-125b by targeting inhibition of BCL2 and MCL1 on multidrug resistance of human gastric cancer cell line
Objective:To investigate the possible role of microRNA-125b(miR-125b) in the development of multidrug resistance(MDR) in human gastric cancer cell line SGC7901 / VCR.Methods:Using quantitative real-time PCR analysis and Western blot to detect the expression difference of miR-125b and anti-apoptotic protein BCL2 and MCL1 between multidrug-resistant human gastric cancer cell line SGC7901 / vincristine(VCR) and its parental [...]