Objective:To study the relationship of CHFR CpG island methylation status on proliferation and apoptosis of esophageal cancer Eca109 cells.Methods: Eca109 cells were treated with different concentrations(2,5,and 10 μmol/L) of 5-Aza-2′-deoxycytidine(5-Aza-CdR),and untreated Eca109 cells were used as control.Methylation-specific PCR(MSP) analysis was used to evaluate the CpG island methylation status of CHFR gene,and RT-PCR was used to [...]
18
May
17
May
pEGFP-N1-DCN suppresses proliferation and invasion of human gastric cancer SGC-7901 cells
Objective To construct an eukaryotic expression vector encoding decorin(DCN)(pEGFP-N1-DCN) and determine its effect on proliferation,cell cycle,migration and invasion of human gastric cancer cell line SGC-7901 after transfection.Methods SGC-7901 cells were divided into 3 groups,control,pEGFP-N1 group and pEGFP-N1-DCN group.SGC-7901 cells were transfected by corresponding plasmids with liposome.The expressions of epidermal growth factor receptor(EGFR) and transforming growth [...]
17
May
Dracorhodin Perchlorate Suppresses Proliferation and Induces Apoptosis in Human Prostate Cancer Cell Line PC-3
The growth inhibition and pro-apoptosis effects of dracorhodin perchlorate on human prostate cancer PC-3 cell line were examined. After administration of 10-80 μmol/L dracorhodin perchlorate for 12-48 h, cell viability of PC-3 cells was measured by MTT colorimetry. Cell proliferation ability was detected by colony formation assay. Cellular apoptosis was inspected by acridine orange-ethidium bromide [...]
17
May
The experimental study of effect of exosomes derived from breast cancer cell line on human umbilical vein endothelial cells
Objective:To observe the effect of exosomes derived from MDA-MB-231 cell line on proliferation,immigration and capillary-like tube formation of Human Umbilical Vein Endothelial cells.Method:Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation,MTT assay was used to observe the effect of exosomes on proliferation of HUVECs;HUVECs were treated with exosomes for 24 h,the change of cell [...]
17
May
Antitumor effects of elemene on the proliferation inhibition and apoptosis induction in ascites hepatoma cell line H22
[Objective]To investigate the antitumor effects of elemene on the proliferation inhibition and apoptosis induction in ascites hepatoma cell line H22.[Methods]Mouse models of the solid tumor of liver cancer were established.The inhibitory rates of solid tumor were observed.The rates of cell viability were observed by MTT.Apoptotic cells were detected by AnnexinV-FITC/PI staining and TUNEL labeling methods.[Results]After [...]
17
May
Effects of Aqueous Extract of Fructus Ligustri Lucidi on the Proliferation and Differentiation of Osteoblastic UMR-106 cells
To study the effects of Fructus Ligustri Lucidi on the proliferation and differentiation of cultured osteoblasts in vitro,the proliferation and the activity of alkaline phosphatase(ALP) in osteoblastic UMR-106 cells was investigated in the presence of water extract of Fructus Ligustri Lucidi(LWE) with Am-blue method and p-nitrophenyl phosphate disodium salt(PNPP) method respectively.The results showed that LWE [...]
16
May
Role of PI3K/Akt and MAPK/ERK pathways in gastric cancer exosome-mediated promotion of tumor cell proliferation
AIM:To investigate the effect of gastric cancer exosomes on homologous tumor cell prolifera-tion and to evaluate the role of PI3K/Akt and MAPK/ERK pathways in this process.METHODS:Exosomes were isolated and purified from human gastric cancer MGC803 cells by serial centrifugation and sucrose gradient ultracentrifugation and observed by electron microscopy.Cell proliferation was measured by MTT assay,and protein [...]
16
May
Effects of histone deacetylase 5 gene on migration of human non-small cell lung cancer
Background and purpose:The acetylation status of histone which is subjected to histone deacetylases(HDACs) and histone acetyltransferases(HATs) regulates the structure of chromatin as well as the expression of genes.The imbalance of HDACs and HATs may contribute to tumorigenesis and tumor development.Histone deacetylase 5(HDAC5) shows low expression in many kinds of tumors.It remains unclear whether or not [...]
16
May
16
May
Effects of Src on Proliferation and Invasion of Lung Cancer Cells
Background and objective It has been proven that Src played pivotal roles in carcinogenesis,cancer progression and metastasis. The aim of this study is to explore the roles of Src phosphorylation on lung cancer cells. Methods Western blot and immunoprecipitation was used to detect the expression and phosphorylation of Src in lung cancer cells. MTT and [...]