Objective To explore the rate change of CD4+ CD25+ regulatory T cells and expression of FOXP3 mRNA after operation in peripheral blood from patients with prostate cancer and its clinical significance.Methods Flow cytometry was used to analyze the proportion of CD4+CD25+ regulatory T cells/CD4+T cells in peripheral blood mononuclear cells(PBMC) among 50 patients with prostate [...]
18
May
17
May
Expression of hPH-20(hSPAM1) mRNA in prostate cancer tissue
Objective To study the expression of human sperm membrane gene hPH-20(hSPAM1)mRNA in the prostate cancer tissue.Methods The hPH-20(hSPAM1)mRNA expression in prostate cancer tissue(n=35) and normal prostate tissue(n=5) were detected by reverse transcriptase polymerase chain reaction(RT-PCR).Results The percentages of hPH-20(hSPAM1)mRNA positive expression in prostate cancer tissue were significantly higher than normal prostate tissue(P<0.05);hPH-20(hSPAM1)mRNA expression in stage [...]
17
May
Value of Contrast-enhanced Ultrasound in Diagnosis of Prostate Cancer
Objective To evaluate the value of contrast-enhanced ultrasound(CEUS)in the diagnosis of prostate cancer(PCA).Methods Atotal of 59 patients with clinically suspected PCA were examined with CEUS and performed transrectal ultrasound guided biopsy of the prostate.The target puncture was implemented at the suspicious region,and were pathologically diagnosed.Contrast enhancement pattern of the prostate nodes were observed and compared [...]
17
May
The diagnosis values of MRI diffusion tensor imaging in prostate disease
Aim:To investigate the value of DTI in diagnosis and differential diagnosis of prostate cancer(PCa) and benign prostatic hyperplasia(BPH).Methods: Twenty-two patients with PCa,31 BPH patients and 20 healthy volunteers were scanned in prostate DTI.All data were transfered to the AW 4.2 workstation for processing.The changes of fractional anisotropy(FA) and average apparent diffusion coefficient(ADC) of PCa foci,BPH [...]
17
May
Dracorhodin Perchlorate Suppresses Proliferation and Induces Apoptosis in Human Prostate Cancer Cell Line PC-3
The growth inhibition and pro-apoptosis effects of dracorhodin perchlorate on human prostate cancer PC-3 cell line were examined. After administration of 10-80 μmol/L dracorhodin perchlorate for 12-48 h, cell viability of PC-3 cells was measured by MTT colorimetry. Cell proliferation ability was detected by colony formation assay. Cellular apoptosis was inspected by acridine orange-ethidium bromide [...]
17
May
Permanent transrectal 125I seeds implantation for analysis of 42 cases prostate cancer
Objective To study the clinical efficacy of 125I brachytherapy combined with surgical castration for treating local advanced prostate cancer.Methods A total of 82 prostate cancer patients were recruited.In treatment group,42 patients with advanced prostate cancer underwent 125I transrectal brachytherapy guided by rectal ultrasound B and orchiectomy at the same time.40 cases of control group were [...]
17
May
16
May
Binding characteristics of interleukin 11 analogue-cyclic nonapeptide c(CGRRAGGSC) to human prostate cancer PC-3 cells:An in vitro study
<Objective:To investigate the binding characteristics of interleukin 11(IL-11) analogue-cyclic nonapeptide c(Cys-Gly-Arg-Arg-Ala-Gly-Gly-Ser-Cys) NH2 C30H54N16O10S2,c(CGRRAGGSC),and human prostate cancer PC-3 cells.Methods: c(CGRRAGGSC) was labeled with fluorescent dye LSS670,and the location of LSS670-cyclic nonapeptide in the PC-3 cells was investigated by fluorescent microscopy.Flow cytometry was used to detect the fluorescence intensity of the in vitro binding of LSS670-c(CGRRAGGSC) to [...]
16
May
Characterization of prostate cancer cell lines and their epithelial-mesenchymal transition in subcutaneous tumors
<Objective:To identify the differences in the expression of epithelial or mesenchymal standard proteins between prostate cancer cell lines and tumors,and to investigate the relationship between the process of the prostate cancer cell line forming subcutaneous tumors and epithelial-mesenchymal transition(EMT) by comparing the characteristics of different prostate cell lines forming subcutaneous tumors in SCID mice.Methods: We [...]
16
May