Objective To provide molecular genetic basis for oncobiological difference in left sided colon cancer and right sided colon cancer.Differentially expressed proteins in left sided colon cancer and right sided colon cancer were screened by proteomic technique.Methods Tissue samples including left sided colon cancer and right sided colon cancer were collected and preserved in the-80℃ refrigerator.In [...]
17
May
16
May
Proteomics of pancreatic cancer
Pancreatic cancer(PC) ranks at the fourth most common cancer-related mortality,and the 5-year survival rate is less than 5%.New biomarkers are needed to improve early diagnosis and develop new effective therapeutic targets.With the development of quantitative proteomics technologies,proteomics technology with better resolution and sensitivity will greatly enhance our capability in understanding the complex molecular events of [...]
15
May
Application of Laser Capture Microdissection in Studies of Gastrointestinal Tumors
Laser capture microdissection(LCM) is a technique that allows the procurement of pure cell populations or single cells from heterogeneous tissue under direct microscopic visualization.By this technique,the limitation of tissue heterogeneity can be solved and cells can be obtained while keeping their natural environment.Therefore,LCM can serve as an indispensable bridge connecting the pathological and molecular biological [...]
15
May
HSP27:a candidate differentially expressed protein between left-and right-sided colon carcinomas
Objective:The aim of the study was to screen differentially expressed proteins between left-and right-sided colon cancers by proteomics techniques and provide molecular genetic basis for oncobiological difference between left-and rightsided colon cancers.Methods:Tissue samples including left-and right-sided colon cancers were collected and preserved in the-80 ℃ refrigeratory.In the first part of our experiment,protein separating was performed [...]
14
May
Chemometrics of differentially expressed proteins from colorectal cancer patients
AIM:To evaluate the usefulness of differentially expressed proteins from colorectal cancer (CRC) tissues for differentiating cancer and normal tissues.METHODS:A Proteomic approach was used to identify the differentially expressed proteins between CRC and normal tissues.The proteins were extracted using Tris buffer and thiourea lysis buffer (TLB) for extraction of aqueous soluble and membrane-associated proteins,respectively.Chemometrics,namely principal component [...]
13
May
Serum proteomic analysis of hereditary nonpolyposis colorectal cancer
AIM:To identify differentially expressed serum proteins between preoperative patients with hereditary nonpolyposis colorectal cancer (HNPCC)and those with sporadic colorectal cancer to find potential markers for the diagnosis of HNPCC.METHODS:The composition of serum proteins was detected by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS)and protein chip assay in 20 preoperative HNPCC patients and 25 patients with [...]
13
May
Separation of Proteome in Human Ovarian Cancer Ascites by Differential Ingel-electrophoresis
Objective To explore and optimize the differential ingel electrophoresis(DIGE) separation of proteome in human ovarian cancer ascites.MethodsBy optimizing the extraction of proteins in human ovarian cancer ascites,parameters on first dimension and SDS-PAGE on second dimension,to determine the method for the separation of proteom.Results The proteins were successfully extracted from human ovarian cancer ascites and were [...]
12
May
Proteomics in cancer research progress
Proteomics as a post-genomics era of the important issues have been extensively to all areas of life sciences,which technology for better diagnosis of cancer provides a good platform,by find a sensitivity and specificity of biomarkers.In this paper,we describes the status and research technology of proteomics,and summaries the areas of proteomics in cancer research briefly.
11
May
Biomarkers of liver cancer related to hepatitis B virus selected by proteomics
Hepatocellular carcinoma(HCC) is one of the most deadly malignancies worldwide.Hepatitis B virus(HBV) is widely recognized as one of the major etiological factors of HCC.However,the mechanism of liver cancer related to HBV infection is still unclear.Liver cancer is difficult to detect in an early stage.Proteomics has caused researcher’s wide attention due to its complementary to other [...]
10
May
Analysis of ovary serous cystadenocarcinoma and borderline cystadenoma using differential-display proteomics
Objective Ovarian serous cystadenocarcinoma(CA) and borderline cystadenoma(IN) of differentially expressed proteins,in order to find specific diagnostic markers.Methods Using two dimensional liquid chromatography MS/MS(2-D LC-MS|MS) technology.Results Identification of CA-IN groups drawn from 96 different proteins,there are 83 differences in protein expression;13 differential protein expression decreased.Conclusion Initially built ovarian serous cystadenocarcinoma protein spectrum of the difference.Cytoskeletal proteins,chaperone [...]