Objectives To study the expression and distribution of PTEN in breast cancer cell MCF-7 after Uv-c irradiation.Methods The cell cycle distribution was examined using flow cytometry.The protein expression was detected with immunoblot analysis.The protein location was detected with immunofluorescence analysis.Results Before UV-c irradiation,the PTEN cells mainly distributed in the cytoplasm surrounding the nucleus;After 24 hours [...]
17
May
12
May
MAGI1 inhibits cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN
Objective To explore the biological function and molecular mechanism of membrane associated guanylate kinase,WW and PDZ domain containing 1(MAGI1) in hepatocellular carcinoma.Methods HepG2MAGI1 stable cell line was constructed by transfecting HepG2 cells with pcDNA3.1-MAGI1 plasmid.Wound healing and invasion assay were performed to compare the migration and invasion ability of HepG2MAGI1 and HepG2 cells.Furthermore,the expression of [...]
10
May
Expression and significance of survivin and PTEN gene in primary hepatocellular carcinoma.
Objective:To investigate the expression of Survivin and PTEN gene and their relationship in primary hepatocellular carcinoma and adjacent tissues.Methods:The expression of Survivin and PTEN were determined in 50 cases of hepatocellular carcinoma and 34 cases of corresponding noncancerous tissues by RT-PCR,and the quantitative detection were followed.Results:Survivin in primary hepatocellular carcinoma is highly expressed in cancer [...]
10
May
Expression and Prognostic Significance of Nuclear PTEN in Gastric Cancer
Objective To evaluate the expression of nuclear PTEN in gastric cancer tissues and identify whether nuclear PTEN was a prognostic factor through analysis for the correlation with clinipathological features.Methods The expression of nuclear PTEN proteins was detected by tissue array and immunohistochemistry in 85 cases of gastric cancer.The relationships between nuclear PTEN expression and their [...]
9
May
Expressions and significances of PTEN and CyclinD1 protein in epithelial tumor of ovary
Objective:To explore the expressions and clinical significances of PTEN and CyclinD1 in epithelial tumor of ovary.Methods:Immunohistochemical method was used to detect the expression levels of PTEN and CyclinD1 protein in 20 cases with normal ovarian tissues,15 cases with benign ovarian tumors and 40 cases with epithelial ovarian cancer.Results:The expression rates of PTEN in normal ovarian [...]
7
May
Growth inhibition of PTEN low expressed breast cancer cells induced by combinational therapy with Lapatinib and Src inhibitor-AZD0530
Objective:To investigate the function of Lapatinib and AZD0530 on PTEN low expressed breast cancer cells.Methods:PTEN-deficient breast cancer cell was created by PTEN knockdown with PTEN-shRNA.After treated with Lapatinib or/and AZD0530,inhibition of cell growth was measured by MTT,cloning formation ability was determined by three-dimensional culture,expression of Akt and Src signaling proteins was analyzed by western blot.Results:Compared [...]
6
May
The expression of survivin and PTEN in cervical cancer and associated research
Objective: To discuss the relationship between survivin and PTEN with the development and invasion of the cancerous tissues.Methods:The expression of survivin and PTEN was investigated using immunohistochemical staining in 100 cases including 48 cases of cervical carcinoma,37 cases of cervical intraepithelial neoplasia(CIN) and 15 cases of normal cervical epithelia.The level of survivin and PTEN expression [...]
5
May
Quantitative analysis of the expression of PTEN protein in lung cancer and its clinical significance
Objective To investigate the expression and clinical significance of PTEN protein in lung cancer.Methods The expressions of PTEN protein in lung cancer,benign pulmonary disease,and normal lung tissue were detected by immunohistochemical SP assay,and were analyzed quantitatively by a Leica Q500MC image analysis system.The single positive intensity(positive unit,PU) was used to quantitatively analyze the expression of [...]
5
May
Recombinant human PTEN suppresses gastric carcinoma cell growth in vitro
Objective:To study the effect of Ad-PTEN gene in SGC-7901tumor cells.To study the inhibition and potential molecular mechanisms of adenovirus-mediated expression of Ad-PTEN gene in SGC-7901 gastric cancer cells.Methods: PTEN gene was transfected into human gastric cancer cell line SGC-7901 with a replication-incompetent adenovirus vector.Using RT-PCR to detect the mRNA expression of PTEN.Using light microscope and [...]
4
May
Relation between HER2 and IGF-IR and PTEN protein expression in breast cancer
Objective To investigate the protein expression of HER2,IGF-IR and PTEN as well as the relationship between these three indicators in breast cancer.Methods IGF-IR and PTEN protein expression was detected by immunohistochemical method in 173 cases of formalin-fixed and paraffin-embedded tumor tissues,including 65 HER2 positive and 108 HER2 negative samples from chinese breast invasive ductal carcinoma [...]