Objective To explore the inhibitory effect of RNA interference(RNAi) targeting human telomerase reverse transcriptase(hTERT) gene of cervical carcinoma Hela cells.Methods Small interfering RNAs(siRNAs) targeting hTERT gene was synthesized with chemical technique,and siRNAs were transfected into Hela cells by cationic lipofection.Cervical carcinoma Hela cells were divided into six groups,blank group(untransfected Hela cells group),negative group(negative control siRNA [...]
18
May
18
May
Effects of Pak1 siRNA to the growth of gastric cancer cell BGC823
Objective To observe the effects of Pak1 siRNA to cell growth and cell cycle of gastric cancer cell BGC823.Methods The expression of Pak1 in gastric cancer cell was detected by Western blot.Pak1 siRNA was transfected into BGC823 cell.The growth ability of BGC823 cell was observed by MTT assay and counting cell number,and the cell cycle [...]
17
May
Construction and Identification of Lentiviral Vector of Double Targeting Regulated by Special Promoter of Liver Cancer and Application of Gene Therapy of Liver
Objective To study the interferencing and anti-tumor effects of lentiviral vector of double Targeting liver Cancer Cell regulated by special survivin-CMV promoter. Methods The fragment of the anti-AFP-scFv was acquired by PCR to recombinant plasmid pMD2G-anti-AFP-scFv. The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed, synthesized and cloned [...]
17
May
Construction and Identification of Eukaryotic Plasmid Expression Vectors Encoding the Short Hairpin RNA Targeting Human GSK-3β and Its Stable Expression in Hum
Objective To construct three short hairpin RNA(shRNA)interference expression plasmid vectors of human GSK-3β gene,detect the expression of GSK-3β in pancreatic carcinoma PANC-1 cells after transfection with recombinant plasmids,and transfect stably the most effective recombinant plasmid into PANC-1 cells.Methods Three plasmid expression vectors coding for shRNA targeting GSK-3β and a control vector were designed.The recombinant plasmids [...]
17
May
Effect of Silencing LRIG3 Gene on the Proliferation and Apoptosis of Bladder Cancer T24 Cells
This study examined the effect of silencing LRIG3 expression on the proliferation and apoptosis of bladder cancer T24 cells and explored the role of LRIG3 in the tumorigenesis of bladder cancer. Bladder cancer T24 cells were routinely cultured and pSilencer plasmids were employed to construct LRIG3 eukaryotic expression vector of LRIG3-siRNA, i.e., pSilencer-LRIG3-siRNA. After confirmation, [...]
17
May
Mechanism of the apoptosis of human gastric cancer cell line SGC7901 induced by vascular endothelial growth factor siRNA
Objective The vascular endothelial growth factor(VEGF),a crucial promoter of blood vessel growth,is over-expressed in many types of tumor tissue.VEGF-targeted siRNA can effectively suppress the proliferation and induce the apoptosis of tumor cells.This study aims to investigate the mechanism of the apoptosis of human gastric cancer cell line SGC7901 induced by VEGF siRNA.Methods SGC7901 cells cultured [...]
17
May
Effects of RNA interference of tissue factor (TF) gene on invasion and metastasis of gastric cancer cells
Objective To investigate the inhibitory effect of short hairpin RNAs(shRNAs) on tissue factor(TF) for affecting the invasion of gastric cancer.Methods According to the principle of RNAi design,pSUPER/TF-siRNA expression vector was constructed,the recombinant plasmid shRNA was transfected into SGC-7901 cells(human gastric cancer cell line) with lipofectamine 2000.The mRNA and protein expression level of TF in SGC-7901 [...]
16
May
Construction and Identification of shRNA Expression Vector for Human Rac1 Gene
Objective To construct the shRNA expression vector for human Rac1 gene and lay a foundation of increasing the sensitivity of ovarian cancer to radiotherapy and chemotherapy.Methods The oligo DNA for construction of shRNA expression vector was designed and synthesized by using shRNA design software according to the sequence of human Rac1 gene in GenBank,based on [...]
16
May
RNA Interference Targeting GRP75 Decreases Cisplatin Resistance in Human Lung Adenocarcinoma Cell
Background and objective GRP75,a member of HSPs which is overexpressed in some resistant cancer cells,is a molecular chaperone mainly located in mitochondrial membrane. The aim of this study is to investigate the role of GRP75 on the resistant mechanisms of cancer cells by downregulating GRP75 expreesion via RNAi approach. Methods Cis-platin-resistant cell A549/CDDP was established [...]
13
May
Selection and optimization of siRNA transfection for targeting EGFR in HepG2 cells by different transfection reagents
Objective To achieve maximum small interference RNA(siRNA) delivery and optimal gene knockdown by testing different siRNA transfection reagents and transfection conditions in the hepatocellular carcinoma(HCC) HepG2 cells,targeting the epidermal growth factor receptor(EGFR).Methods The siRNA targeting wild type EGFR was designed and siRNA transfection was performed with different methods to achieve the best transfection efficiency in [...]