MicroRNAs are post-transcriptional regulators,composed by approximately 21 ribonucleic acid (RNA) molecules.By binding to complementary sequences of target genes,they can cause degradation of the mRNAs of target genes or inhibit their translation.MicroRNAs have been implicated in processes and pathways such as development,cell proliferation,apoptosis,metabolism and morphogenesis,and in diseases including cancer.This paper aimed to introduce achieving silencing of [...]
18
May
17
May
Construction of colon cancer HT-29 cell line with αv integrin stably silenced
Objective To explore efficiency and specificity of gene silence by retroviral delivery of short hairpin RNA(shRNA) targeting for αv integrin and to construct colon cancer HT-29 cell line with αv integrin stably silenced.Methods Three pairs of specific DNA sequences encoding shRNA targeting for αv integrin were synthesized.Retroviral vectors expressing αv integrin specific shRNA were constructed [...]
16
May
STAT2 shRNA Inhibits HeLa Cell Proliferation
Recently it was reported that signal transducer and activator of transcription 2(STAT2) involves in carcinogenesis. Previous study showed that several kinds of tumor tissues as well as tumor cell lines highly expressed STAT2. Therefore,in order to further explore the effects of STAT2 on carcinogenesis,STAT2 expression level in cervical cancer cell line HeLa was decreased by [...]
16
May
12
May
Mechanisms of EGFR and IGF-1R Blockade by siRNA Therapy for Growth Control of Hepatocellular Cancer HepG2 Cells
Objective: To investigate the antineoplastic effects of inhibiting IGF-1R and EGFR and signaling in hepatocellular cancer(HCC) HepG2 cells by siRNA simultaneous blockade of both of IGF-1R and EGFR.Methods: The growth,cell cycle progression,cellular apoptosis,and the related proteins including bcl-2,cdk2,bax and P21 were analyzed by MTT,flow cytometry,and Western blot.Results: Forty-eight hours after transfection,silencing both of EGFR and [...]
12
May
Fibronectin EDA domain regulates proliferation and apoptosis of colorectal cancer SW480 cells
Objective To determine the effect of fibronectin extra type Ⅲ domain A(EDA-FN) on the proliferation and apoptosis of human colorectal cell line SW480.Methods The expression of EDA-FN in SW480 cells was silenced by lentiviral infection.Expression of EDA-FN was examined by Western blot analysis.MTT assay was used to analyze cell viability after infection.Cell apoptosis was analyzed [...]
11
May
shRNA interference of Kaiso gene has an influence on the biological behaviour of human lung cancer cells
Objective Kaiso,a member of the BTB/POZ protein family,regulates several important genes,some of which are invasion and proliferation-related.In present study,we employed shRNA(small hairpin RNA) technology to down-regulate nuclear Kaiso expression and to investigate the the biological behavior of lung cancer cells.Methods Kaiso expression was specifically interfered by shRNA in LTE and SPC cells.Immunofluorescence was used to [...]
10
May
Vector-mediated RNAi in cancer therapy research
RNA interference(RNAi) induced by double-strand RNA is a process of post-transcriptional gene silence.The exogenous siRNAs are led into the host cell by vector,and regulate gene expression on and off,which provide a new and effective way for cancer treatment.In this paper,vector-mediated RNAi in cancer therapy was reviewed.
8
May
Construction of expressive system of DNA methyltransferase 1 RNAi baculovirus
Objective: To build recombinant baculovirus carrier which carried with DNA methyltransferase(DNMT)1 RNAi in order to further study the influrence of DNMT1 to pancreatic cancer related genes.Methods: First annealed 60 bp sense oligodeoxynucleotide(SODN) and antisense oligodeoxynucleotide(ASODN) in the cohesive end with BglⅡ and Hind Ⅲ restriction endonuclease,linked with linearizing carrier pSUPER to build pSUPER si-DNMT1-D.Then cut [...]
8
May
Current status and development of miRNA and siRNA research on gastric cancer
RNA interference(RNAi) is an important topic of epigenetics research in post-genome period.RNAi works as a post-DNA replication regulator for gene expression,and it is related to the occurrence and development of malignant tumors.The most usual participators of RNAi are MicroRNA(miRNA) and small interference RNA(siRNA).This review summarizes the basic theory of miRNA and siRNA,and provides recent progresses [...]