Objective To study the expression of human sperm membrane gene hPH-20(hSPAM1)mRNA in the prostate cancer tissue.Methods The hPH-20(hSPAM1)mRNA expression in prostate cancer tissue(n=35) and normal prostate tissue(n=5) were detected by reverse transcriptase polymerase chain reaction(RT-PCR).Results The percentages of hPH-20(hSPAM1)mRNA positive expression in prostate cancer tissue were significantly higher than normal prostate tissue(P<0.05);hPH-20(hSPAM1)mRNA expression in stage [...]
17
May
17
May
Expression of two transcriptional splice variants CK1αLS and CK1αS of human CSNK1A1 gene in cell lines
Aim:To detect the functions of casein kinase 1alpha 1(CSNK1A1 or CK1α)gene on carcinogenesis and development by studying the expression of two transcriptional splice variants CK1αLS and CK1αS of CK1α gene in normal cell lines and malignant cell lines.Methods: The mRNA expression levels of CK1αLS and CK1αS of CSNK1A1 gene were examined by RT-PCR in thirty-six [...]
17
May
Expression of Bmi-1 in NSCLC and Its Relationship to Clinicopathologic Characters
Objective:To investigate the relationship between the expression of Bmi-1 in nonsmall-cell lung cancer(NSCLC) and clinicopathologic characters.Methods:Reverse transcription-PCR(RT-PCR)was applied to detect Bmi-1 mRNA in 30 of NSCLC speci-men and 20 of tumor-adjacent tissue.Immunohistochemistry was performed to detect the expression of Bmi-1 in 52 of NSCLC specimen and 30 of tumor-adjacent tissue.Results:The expression of Bmi-1mRNA in NSCLC [...]
16
May
Study about expression of proteinase activated receptor-2 in human pancreatic cancer cells and its related mechanism
AIM:By observing expression of PAR-2 in human pancreatic cancer cells and cancer tissue adjacent.In order to clear of expression characteristic of PAR-2 in pancreatic cancer and explore relevant mechanism.METHODS: Collect pancreatic specimens and adopting method of SP to detect expression of pancreatic cancer PAR-2 gene.Cultivate pancreas cancer cells and adopting RT-PCR detection expression of PAR-2 [...]
13
May
Effect of Dys-psychological Stress on Expression of Dicer in Mice with Human Ovarian Cancer Xenografts
Objective To observe the effect of dys-psychological stress on the expression of dicer in mice with human ovarian cancer xenografts.Methods The subcutaneous tumor xenografts were established by implanting human ovarian cancer tissue into nude mice.After establishment of the model,the mice with similar weight and diameter of tumors were randomly divided into control group and stress [...]
11
May
The RT-PCR detection of retinoblastoma binding protein 8 in esophageal cancer cells
Aim To detect the differential expression of retinoblastoma binding protein 8(STRBP8) in human epithelial cells of esophageal cancer and provide evidence for identification of specific biomarkers and regulatory mechanisms involved in the pathogenesis of esophageal cancer.Methods The corresponding primers were designed according to the CDS sequence of STRBP8 in GenBank,which were used to amplify STRBP8 [...]
11
May
The study on the expression of IGF-IR and its protein in human urinary bladder cancer
Objective To detect the expression of IGF-IR and its protein in human urinary bladder cancer,to evaluate its role in the carcinogenesis and progression of tumor and clinical significance.Methods Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) and immunohistochemistry were used to detect the expression of IGF-IR and its proten in 30 urinary bladder cancer and the corresponding [...]
11
May
Study on effect of histone deacetylase inhibitor SAHA combined with radiotherapy on cervical cancer cells
Objective:To explore the effect of histone deacetylase inhibitor SAHA combined with radiotherapy on human cervical cancer SiHa cells.Methods:The cell growth inhibition ratio of different concentrations of SAHA were assessed by MTT assay at 48 h,and then the IC50 were calculated.The inhibition rate of 20% IC50 of SAHA was selected with radiotherapy.Cases were divided into four [...]
10
May
Relationship between the Over-expression of HSP70 Gene and Human Breast Cancer and Cervical Cancer
Objective To study the expression of HSP70 gene from tissues of breast cancer and cervical cancer.Methods Tissue samples of cancers (breast cancer,cervical cancer)were obtained from operating room of Baoan People’shospita1.RNA was extracted according to the cyanic acid guanidine law of different sulphur separately.RNA separation carried out with 1% agarose electrophoresis.RT-PCR was performed to analyze the [...]
10
May
Expression and significance of survivin and PTEN gene in primary hepatocellular carcinoma.
Objective:To investigate the expression of Survivin and PTEN gene and their relationship in primary hepatocellular carcinoma and adjacent tissues.Methods:The expression of Survivin and PTEN were determined in 50 cases of hepatocellular carcinoma and 34 cases of corresponding noncancerous tissues by RT-PCR,and the quantitative detection were followed.Results:Survivin in primary hepatocellular carcinoma is highly expressed in cancer [...]