In this studv by usine immunohistochemistry stain method Survivin and Caspase-3were detected in the colonic cancer tissue and normal tissue of 42patients,and colonic adenoma tissue of 38patients so as to investigate the expressions of the two proteins and their significance.As results,the positive expression rate of Survivin in the tissue of colonic cancer was significantly higher [...]
18
May
17
May
Study on expressions of survivin and p53 in epithelial ovarian cancer
Objective To explore the expression of survivin and p53 in epithelial ovarian cancer and their clinical significances.Methods Expression intensity of survivin and p53 were detected by immunohistochemistry in the tissues of 46 cases with epithelial ovarian cancer,the relationship between clinical and pathological characteristics with survivin and p53 expressions were analysed.The 44 cases with benign epithelial [...]
17
May
Effect and clinical significance of survivin expression in intra-uterine arterial neoadjuvant chemotherapy for stage Ⅱ cervical carcinoma
Objective To evaluate the effects and clinical significance of survivin expression in intra-uterine arterial neoadjuvant chemotherapy for stage Ⅱ locally advanced cervical cancer.Methods Seventy-seven patients with locally advanced cervical cancer,FIGO Stage Ⅱ,from January 2005～December 2007 were enrolled in this study,those patients were primary treatment by neoadjuvant intra-uterine arterial chemotherapy.Immunohistochemistry were performed to eximine the expression [...]
16
May
The Expression and Its Significance of AIF、HSP70 and Survivin in Gastric Cancer
Objective:To study the expression of AIF,HSP70 and survivin in gastric cancer and to study the relationship with development of gastric cancer.Methods:Immunohistochemistry staining method was used to examine the expression of AIF,HSP70 and survivin in 60 cases of gastric cancer and 60 normal control gastric tissues.Results:The score of AIF,HSP70 and survivin in gastric cancer,compared with normal [...]
16
May
Expression and clinic value of Survivin,MMP-2,nm23-H1,VEGF and Flt-1 in colorectal carcinomas
Background and purpose:The aim of this study was to investigate the relationship between the expression of Survivin,MMP-2,nm23-H1,VEGF,Flt-1 and clinicopathologic parameters in colorectal carcinoma(CRC).Methods:The expression of Survivin,MMP-2,nm23-H1,VEGF and Flt-1 were detected using the immunohistochemical method in 72 CRCs and their match normal tissue around tumor.The correlations between the expression of Survivin,MMP-2,nm23-H1,VEGF,Flt-1 and clinicopathologic parameters were analyzed.Results:The [...]
16
May
Survivin is involved in drug resistance of pancreatic cancer PaTu8988 cells to gemcitabine
Objective:To explore the expression of apoptotic inhibitory protein survivin in pancreatic cancer cell line PaTu8988,and to study its role in the drug resistance of PaTu8988 cells to gemcitabine(GEM).Methods: The inhibitory effect of GEM(0.01,0.1,1.0,2.5,5.0,and 10.0 μg/ml) on PaTu8988 cells was detected by MTT assay;apoptosis rate of PaTu8988 cells treated with GEM was determined by flow cytometry;and [...]
15
May
Expression and clinical significance of Survivin and Runx3 in gastric carcinoma
Objective:To study the expression and clinical significance of Survivin and Runx3 in gastric carcinoma tissue.Methods:The expressions of Survivin and Runx3 were detected by SP immunohistochemical technique in 52 cases of gastric carcinoma and analyze the relationship between the expression of Survivin,Runx3 and the clinicopathological character of samples.Results:The expression rate of Runx3 in gastric cancer was [...]
15
May
The expression and significance of cyclin B1 and survivin in human non-small cell lung cancer
Objective:We studied the expression of cyclin B1 and survivin in human non-small cell lung cancer(NSCLC),and the relationship between such expression and clinicopathological features of NSCLC.Methods:One hundred cases of tissue specimen including NSCLC,neighboring noncancerous tissue and normal lung tissue were collected at random.These specimens were detected by immunohistochemical methods.Results:The expression of cyclin B1 and survivin showed [...]
13
May
Study of human cervical cancer Hela Apoptosis induced by saikosaponin D
Objective To investigate the effect of Saikosaponin D on apoptosis in human cervical cancer Hela cell line and its detailed mechnisms.Methods Human cervical cancer Hela cells was treated by Saikosaponin D with various concentrations(0、2.5、5、7.5μg/ml respectively),then the cell viability was determined by MTT assay at different time.After treatment for 48h,apoptosis rate was determined by flow cytometry.Cell [...]
13
May
Expressions and correlation of survivin,FHIT and VEGF-C in gastric adenocarcinoma
Objective To investigate the expressions and correlation of survivin,fragile histidine triad(FHIT) and vascular endothelial growth factor C(VEGF-C) in gastric adenocarcinoma.Methods The expressions of survivin,FHIT and VEGF-C mRNA were detected by semi-quantitative RT-PCR in cancer tissues,tumor-adjacent normal tissues and distal normal tissues of 43 gastric adenocarcinoma cases.Results Compared with tumor-adjacent normal tissues and distal normal tissues,the [...]