Objective:To investigate the expression of vascular endothelial growth factor C(VEGF-C) and Vascular endothelial growth factor receptor-3(VEGFR-3) in gastric cancer,so that to determine the molecular markers in prognosis of gastric cancer.Methods:Collecting clinical data methods,Real-time PCR and ELISA were used to detect the expression of VEGF-C and VEGFR-3 in 43 cases of gastric cancer.Results:43 cases of gastric [...]
17
May
17
May
Mechanism of the apoptosis of human gastric cancer cell line SGC7901 induced by vascular endothelial growth factor siRNA
Objective The vascular endothelial growth factor(VEGF),a crucial promoter of blood vessel growth,is over-expressed in many types of tumor tissue.VEGF-targeted siRNA can effectively suppress the proliferation and induce the apoptosis of tumor cells.This study aims to investigate the mechanism of the apoptosis of human gastric cancer cell line SGC7901 induced by VEGF siRNA.Methods SGC7901 cells cultured [...]
16
May
ANXA-2 and VEGF promote invasiveness of human liver cancer HepG2 cells
AIM:To investigate the role of annexin-2(ANXA-2)and vascular endothelial growth factor(VEGF)in promoting invasiveness of human liver cancer HepG2 cells.METHODS:After HepG2 cells were treated with different doses of 5-fluorouracil,cell invasiveness was detected by Transwell assay,and the mRNA and protein expression of ANXA-2 and VEGF was detected by RT-PCR and Western blot,respectively.RESULTS:The invasiveness of HepG2 cells decreased with [...]
16
May
Expression and clinical significance of LOX-5 and VEGF in human breast cancer
Objective To investigate the expression of LOX-5 and VEGF in breast cancer and analyze the effect of LOX-5,VEGF in breast cancer development and its relationship with olinicopathological factors.Methods LOX-5 and VEGF expression were detected by immunohistochemistry in 60 cases of breast cancer,20 cases of breast fibroadenoma and 20 cases of adjacent normal tissue.Correlation with the [...]
16
May
Clinical values of detecting soluble CD44v6 and VEGF in differentiate tuberculous ascites and ascites in ovarian can cer
Objective To investigate the diagnostic value of soluble CD44v6(sCD44v6) and VEGF in the tuberculous ascites and ovarian cancer ascites.Methods Used enzyme linked immunosorbent assay(ELISA) to detect sCD44v6 and VEGF levels in 39 cases of ovarian cancer ascites,and 48 cases of tuberculous ascites.Results sCD44v6 was(102±31 ) ng/ml in ovarian cancer ascites group,which was significantly higher than [...]
16
May
Expression and Implication of VEGF in Ovarian Cancer
Objective To study the relationship of vascular endothelial growth factor(VEGF) expression level and the clinical stages in ovarian cancer.Methods Thirty-three patients with ovarian cancer were enrolled as the experiment group and nine patients with normal uterine endometria were taken as the control group.Immunohistochemistry was used to detect the VEGF levels in the two groups.Results The [...]
16
May
Relation of advanced oxidation protein products with VEGF and TGF-β1 in colon cancer cells exposed to intermittent hypoxia
Objective To investigate the association of advanced oxidation protein produts(AOPP) with oxidative stress in colon cancer cells exposed to intermittent hypoxia(IH).Methods Colon cancer SW480 cells were exposed to IH,continuous hypoxia,or normoxia.Enzyme-linked immunosorbent assay(ELISA) was employed to examine the levels of AOPP and vascular endothelial growth factor(VEGF),xanthine oxidase assay was used to determine malonaldehyde(MDA) and glutathione [...]
16
May
The relationship between TSP-1、VEGF And relapse and metastasis after surgery of breast cancer tissue
Objective To study the role of TSP-1 and VEGF in the process of breast cancer infiltration,metastasis,relapse and prognosis.Methods 180 cases of breast cancer were followed up.The relationship between the expression of TSP-1 and VEGF and the tumor relapse and metastasis were studied.Results The relapse rate of TSP-1 positive expression was lower than it of TSP-1 [...]
15
May
CD146,VEGF and their correlations with angiogenesis in prostate cancer
Objective To study the expression of CD146 and VEGF in prostate cancer tissue and their relationship with tumor angiogenesis.MethodsImmunohistochemical SP method was used to detect the expression of CD146 and VEGF in 39 cases of prostate cancer tissue and 20 cases of benign prostatic hyperplasia(BPH)tissue,the microvessel density(MVD)marked by CD34 was also detected.ResultsThe positive expression rate [...]
15
May
Study on expression of Notch signal pathway and VEGF in human non-small cell lung cancer
Objective To research the expressions of Notch 1,Delta 4 and vascular endothelial growth factor(VEGF) in human non-small cell lung cancer(NSCLC) and to explore its clinical significance.Methods Immunohistochemical SP method was used to detect the expressions of Notch 1,Delta 4 and VEGF in these 55 NSCLC tissues and 30 normal epithelial tissues of the lung.The RT-PCR [...]