Objective:To evaluate the Effects of xiaoliu decoction combined with hyperthemic peritoneal perfusion chemotherapy on immune function and the protein expression of serum vascular endothelial growth factor in Colorectal cancer after surgery treated.Methods:104 cases with surgical treatment of patients with advanced colorectal cancer were randomly divided into two groups:Treatment Group of 54 patients used to intraperitoneal [...]
16
May
16
May
Expression and clinic value of Survivin,MMP-2,nm23-H1,VEGF and Flt-1 in colorectal carcinomas
Background and purpose:The aim of this study was to investigate the relationship between the expression of Survivin,MMP-2,nm23-H1,VEGF,Flt-1 and clinicopathologic parameters in colorectal carcinoma(CRC).Methods:The expression of Survivin,MMP-2,nm23-H1,VEGF and Flt-1 were detected using the immunohistochemical method in 72 CRCs and their match normal tissue around tumor.The correlations between the expression of Survivin,MMP-2,nm23-H1,VEGF,Flt-1 and clinicopathologic parameters were analyzed.Results:The [...]
14
May
The application of vascular endothelial growth factor testing in diagnosis of colon cancer
Objective: To explore the application of vascular endothelial growth factor(VEGF) testing in diagnosis of colon cancer.Methods: A total of 60 cases with colon cancer admitted from January 2008 to April 2010 were selected as the observation group,and 30 healthy volunteers from our outpatient were selected as control group.The level of VEGF expression was tested by [...]
13
May
The expression of TP,VEGF and its relationship with clinical Pathological significance in Colorectal Cancer
Objective To investigate the expression of thymidine phosphorylase(TP) and vascular endothelial growth factor(VEGF) and its relationship with clinical pathological charucteristies of colorectal cancer.Methods Immunohistochemical staining method EnVision TM was used to investigate the expression of TP and VEGF in 88 cases of colorectal cancer and its relationship with clinical pathology.Results TP and VEGF were not [...]
13
May
Expression and clinical significance of CTGF,VEGF and HIF-1α in pancreatic carcinoma
Objective: To investigate the expressions of CTGF,VEGF,HIF-1α and the relationships between them and various clinical pathological characteristics in human pancreatic carcinoma.Methods: To investigate the expressions of CTGF,VEGF and HIF-1α in tumor issues from 46 radically resected specimens of pancreatic carcinoma and 10 normal pancreatic tissues by Envision two-step immunohistochemical staining.Results: CTGF,VEGF and HIF-1α in pancreatic [...]
12
May
Inhibition of VEGF expression by shRNA and effect on the apoptosis of cervical cancer SiHa cell line
Objective To study the induction of apoptosis by RNA interference-mediated inhibition of VEGF expression in SiHa cells.Methods Two types of short hairpin RNA were designed and prepared.The recombinant plasmid PGPU6/GFP/Neo-VEGF were constructed respectively,named pv1 and pv2,which were transfected into SiHa cells using LipofectamineTM 2000.RT-PCR was performed to detect the expression of VEGF mRNA.The most efficient [...]
12
May
Expression and clinical pathological significance of VEGF,KAI-1,c-kit in triple-negative breast cancer
Objective To explore the expression and clinical pathological significance of VEGF,KAI-1,c-kit in triple-negative breast cancer.Methods PV-9000 immunohistochemistry technique was applied to detect the expression of VEGF,KAI-1,c-kit in 85 triple-negative breast cancer cases.Results (1) The positive rate of VEGF was 76.47%(65/85) in TNBC.The expression of VEGF was associated with tumor size(P<0.05).(2) The positive rate of KAI-1 [...]
11
May
CA153、CA125 And VEGF for the Clinical Evaluation before and after Breast Cancer Surgery
Objective:To evaluate the prognosis of clinical significance of CA153,CA125 and VEGF in breast cancer patients before and after surgical treatment.Methods:From January 2007 to January 2009 in our hospital the female breast surgery treated patients with invasive ductal carcinoma of 131 patients,comparing all patients before and 3 months after follow-up,serum CA153,CA125 and VEGF values change. Results:The [...]
11
May
Mechanisms of Thalidomide Action in Gallbladder Cancer Cells
Objective To investigate the mechanisms of Thalidomide action in gallbladder cancer(GBC) cells.Methods RT-PCR was employed to examine the expression of survivin,VEGF and MMP-9 in GBC-SD cells.Results The expression of survivin,VEGF and MMP-9 in GBC-SD cells was decreased by Thalidomide,and the expression was related to the concentration of Thalidomide.Conclusion Thalidomide can inhibit the growth of GBC [...]
11
May
Expression of MIM and VEGF and their prognostic significance in gastric carcinoma
【Objective】 To investigate expression of MIM(missing in metastasis) and VEGF(vascular endothelial growth factor) and their prognostic significance in gastric carcinoma.【Methods】 Tissue microarray blocks containing gastric tumor specimens from 282 patients were constructed.Expressions of MIM and VEGF in these specimens were analyzed using immunohistochemical(IHC) studies.【Results】 IHC showed that negative expression rate of MIM in gastric cancer [...]