Objective To investigate the expression of carbonic anhydrase Ⅱ(CAⅡ) in colorectal cancer(CRC),and the correlation with the carcinogenesis,development and clinical pathological aspects of CRC.Methods CAⅡ was detected in paired fresh samples of 12 CRC and the mucosa distant to cancer(normal mucosa) with Western Blot.Then the expression of CAⅡ was detected in paired samples of 64 CRC [...]
17
May
15
May
Express significance of insulin like growth factor receptor-1 in endometrial cancer tissue
Objective To evaluate the level of Insulin like growth factor(IGF)in endometrial cancer tissue and to study the relationship between Insulin like growth factor receptor-1(IGF-1R)and endometrial cancer genesis.Methods The protein level of IGF-1R from endometrial cancer tissue in 30 patients with endometrial cancer and that from endometrial tissue in 20 cases resected uterine for benign of [...]
13
May
Analysis and clinical significance of ZO-1 protein in patients with NSCLC
Objective:The objective of this study was to investigate the zonula occluden-1(ZO-1) protein in patients with Non Small Cell Lung Cancer(NSCLC) and to indentify this roles in pathogenesis,development and classification of NSCLC.Methods: The ZO-1 protein of 101 patients with NSCLC and 61 patients with benign lung disease were detected by western blot.Results: There was significant statistic [...]
5
May
Construction of the eukaryotic expressing vector of human PAK2 gene and the expression and location in gastric cancer cells
Objective To construct an eukaryotic expressing vector of human PAK2 gene and identify its recombinant plasmid expression and location in the gastric cancer cells.Methods Total mRNA was extracted from HeLa cells,cDNA was formed by reverse transcription.The PAK2 coding sequence was amplified by polymerase chain reaction(PCR)method and cloned into pcDNA3.1A vector.After the target region was sequenced,the [...]
4
May
Expression of UFC1 Gene in Breast Cancer Tissues and Normal Breast Tissues
To investigate the expression of UFC1(ubiquitin-fold modifier conjugating enzyme 1) in breast cancer tissues and adjacent normal breast tissues,and analyze its relationship with clinicopathologic characteristics.The expressions of UFC1,in 20 cases of breast cancer tissues and matched normal breast tissues,were examined by RT-PCR,Western-blot and immuno-histochemistry.The correlation of UFC1 expression level with clinicopathologic characteristics was analyzed.The expression [...]
4
May
Relation between Enhancer of Zeste Homolog 2 and Cisplatin Resistantance of Human Epithelial Ovarian Cancer Cells
Objective To study enhancer of zeste homolog 2(EZH2) mRNA and protein expression in human ovarian cancer cell line A2780 and Cisplatin resistant ovarian cancer cell strain A2780/DDP,and discuss the correlation between EZH2 and Cisplatin resistance in ovarian cancer.Methods Expression of EZH2 mRNA in A2780 and A2780/DDP were tested by reverse transcription-polymerase chain reaction(RT-PCR).EZH2 protein contents [...]
4
May
3
May
Construction of hFAK Gene-recombinant Plasmids and its Protein Expression
Objective To construct the recombinant plasmids of human hFAK gene and identify its protein expression.Methods Total RNA was extracted from human gastric cancer SGC-7901 cells.The hFAK coding sequence was amplified by polymerase chain reaction(PCR)method and cloned into pCDNA3.1/Flag and pGEX-4T-2 vectors respectively.IPTG-induced GST-hFAK protein expression in BL21 cells and protein purification were identified by SDS-PAGE.The [...]
3
May
Searching the breast cancer serum marker by using the comparative proteomics technology
Early diagnosis is a one of the key to improve the breast cancer patients’ prognosis.To investigate the breast cancer serum-biomarkers,high-abundant protein was depleted from 400(portion) breast cancer patients’ serum and 40 healthy control serum.The enriched proteins were then separated by 2D-PAGE(two-dimensional polyacrylamide gel electrophoresis) and the gels were dyed by silver-stain,then three different proteins were [...]
30
Apr
Expression of sodium/iodide symporter in different thyroid tissue
Objective: To detect the expression of sodium/iodide symporter(NIS)in different thy-roid tissue,and the distribution of NIS in papillary thyroid carcinoma.Methods: sixteen cases of normal thyroid tissue,14 cases of nodular goiter and 23 cases of papillary thyroid carcinoma were selected,Western blot technique were used to detecte NIS expression in various tissues.Then,cytoplasm and membrane proteins of papillary thyroid [...]